Combined analysis of the salivary microbiome and host defence peptides predicts dental disease

Simon-Soro, A., Sheriff, A., Sadique, S., Ramage, G. , Macpherson, L. , Mira, A., Culshaw, S. and Malcolm, J. (2018) Combined analysis of the salivary microbiome and host defence peptides predicts dental disease. Scientific Reports, 8, 1484. (doi:10.1038/s41598-018-20085-x)

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Abstract

Understanding the triad of host response, microbiome and disease status is potentially informative for disease prediction, prevention, early intervention and treatment. Using longitudinal assessment of saliva and disease status, we demonstrated that partial least squares modelling of microbial, immunological and clinical measures, grouped children according to future dental disease status. Saliva was collected and dental health assessed in 33 children aged 4 years, and again 1-year later. The composition of the salivary microbiome was assessed and host defence peptides in saliva were quantified. Principal component analysis of the salivary microbiome indicated that children clustered by age and not disease status. Similarly, changes in salivary host defence peptides occurred with age and not in response to, or preceding dental caries. Partial least squares modelling of microbial, immunological and clinical baseline measures clustered children according to future dental disease status. These data demonstrate that isolated evaluation of the salivary microbiome or host response failed to predict dental disease. In contrast, combined assessment of both host response together with the microbiome revealed clusters of health and disease. This type of approach is potentially relevant to myriad diseases that are modified by host–microbiome interactions.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ramage, Professor Gordon and Macpherson, Professor Lorna and Malcolm, Dr Jennifer and Culshaw, Dr Shauna
Authors: Simon-Soro, A., Sheriff, A., Sadique, S., Ramage, G., Macpherson, L., Mira, A., Culshaw, S., and Malcolm, J.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:Scientific Reports
Publisher:Nature Publishing Group
ISSN:2045-2322
ISSN (Online):2045-2322
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in Scientific Reports 8:1484
Publisher Policy:Reproduced under a Creative Commons License

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