Development of a novel equine influenza virus live-attenuated vaccine

Rodriguez, L., Reedy, S., Nogales, A., Murcia, P. R. , Chambers, T. M. and Martínez-Sobrido, L. (2018) Development of a novel equine influenza virus live-attenuated vaccine. Virology, 516, pp. 76-85. (doi: 10.1016/j.virol.2018.01.005) (PMID:29331866) (PMCID:PMC5840510)

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Abstract

H3N8 equine influenza virus (EIV) is an important and significant respiratory pathogen of horses. EIV is enzootic in Europe and North America, mainly due to the suboptimal efficacy of current vaccines. We describe, for the first time, the generation of a temperature sensitive (ts) H3N8 EIV live-attenuated influenza vaccine (LAIV) using reverse-genetics approaches. Our EIV LAIV was attenuated (att) in vivo and able to induce, upon a single intranasal administration, protection against H3N8 EIV wild-type (WT) challenge in both a mouse model and the natural host, the horse. Notably, since our EIV LAIV was generated using reverse genetics, the vaccine can be easily updated against drifting or emerging strains of EIV using the safety backbone of our EIV LAIV as master donor virus (MDV). These results demonstrate the feasibility of implementing a novel EIV LAIV approach for the prevention and control of currently circulating H3N8 EIVs in horse populations.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Murcia, Professor Pablo
Authors: Rodriguez, L., Reedy, S., Nogales, A., Murcia, P. R., Chambers, T. M., and Martínez-Sobrido, L.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Virology
Publisher:Elsevier
ISSN:0042-6822
ISSN (Online):1096-0341
Published Online:11 January 2018
Copyright Holders:Copyright © 2018 Elsevier Inc.
First Published:First published in Virology 516:76-89
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
709011Quinquennial Core FundsMassimo PalmariniMedical Research Council (MRC)MC_UU_12014/9MVLS III - CENTRE FOR VIRUS RESEARCH