Insulin aggregation tracked by its intrinsic TRES

Chung, L. H. C., Birch, D. J.S., Vyshemirsky, V. , Ryadnov, M. G. and Rolinski, O. J. (2017) Insulin aggregation tracked by its intrinsic TRES. Applied Physics Letters, 111(26), 263701. (doi: 10.1063/1.5008477)

Abstract

Time-resolved emission spectra (TRES) have been used to detect conformational changes of intrinsic tyrosines within bovine insulin at a physiological pH. The approach offers the ability to detect the initial stages of insulin aggregation at the molecular level. The data analysis has revealed the existence of at least three fluorescent species undergoing dielectric relaxation and significant spectral changes due to insulin aggregation. The results indicate the suitability of the intrinsic TRES approach for insulin studies and for monitoring its stability during storage and aggregation in insulin delivery devices.

• Item Type: Articles
• Additional Information: This work was supported in part by the UK’s Department for Business, Energy and Industrial Strategy.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Vyshemirsky, Dr Vladislav
• Authors: Chung, L. H. C., Birch, D. J.S., Vyshemirsky, V., Ryadnov, M. G., and Rolinski, O. J.
• College/School: College of Science and Engineering > School of Mathematics and Statistics > Statistics
• Journal Name: Applied Physics Letters
• Publisher: AIP Publishing
• ISSN: 0003-6951
• ISSN (Online): 1077-3118
• Published Online: 27 December 2017
• Copyright Holders: Copyright © 2017 AIP Publishing
• First Published: First published in Applied Physics Letters 111(26): 263701
• Publisher Policy: Reproduced in accordance with the publisher copyright policy