Ramirez, M. I. et al. (2018) Technical challenges of working with extracellular vesicles. Nanoscale, 10(3), pp. 881-906. (doi: 10.1039/c7nr08360b) (PMID:29265147)
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Abstract
Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.
Item Type: | Articles |
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Additional Information: | The authors acknowledge the support received from the British Council/Newton Fund (203855854), Fundação de Amparo à Pesquisa do Estado de São Paulo (2012/24574-3; 2015/50257-3; 2014/26897-0; 2014/06863-3), Associação Beneficente Alzira Denise Hertzog Silva (ABADHS), Conselho Nacional de Desenvolvimento Científico e Tecnológico (441878/ 2014-8; 202077/2015-2), Brazil; Ricerca Finalizzata (Convenzione no. 172/GR-2011-02350301) from the Ministero della Salute, Italy; Science without Borders/CAPES, Brazil (BEX 7057/15-6). MIR and ED-N are research fellows from CNPq, Brazil. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Coakley, Miss Gillian |
Authors: | Ramirez, M. I., Amorim, M. G., Gadelha, C., Milic, I., Welsh, J. A., Freitas, V. M., Nawaz, M., Akbar, N., Couch, Y., Makin, L., Cooke, F., Vettore, A. L., Batista, P. X., Freezor, R., Pezuk, J. A., Rosa-Fernandes, L., Carreira, A. C. O., Devitt, A., Jacobs, L., Silva, I. T., Coakley, G., Nunes, D. N., Carter, D., Palmisano, G., and Dias-Neto, E. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | Nanoscale |
Publisher: | Royal Society of Chemistry |
ISSN: | 2040-3364 |
ISSN (Online): | 2040-3372 |
Published Online: | 13 December 2017 |
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