Technical challenges of working with extracellular vesicles

Ramirez, M. I. et al. (2018) Technical challenges of working with extracellular vesicles. Nanoscale, 10(3), pp. 881-906. (doi: 10.1039/c7nr08360b) (PMID:29265147)

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Abstract

Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.

Item Type:Articles
Additional Information:The authors acknowledge the support received from the British Council/Newton Fund (203855854), Fundação de Amparo à Pesquisa do Estado de São Paulo (2012/24574-3; 2015/50257-3; 2014/26897-0; 2014/06863-3), Associação Beneficente Alzira Denise Hertzog Silva (ABADHS), Conselho Nacional de Desenvolvimento Científico e Tecnológico (441878/ 2014-8; 202077/2015-2), Brazil; Ricerca Finalizzata (Convenzione no. 172/GR-2011-02350301) from the Ministero della Salute, Italy; Science without Borders/CAPES, Brazil (BEX 7057/15-6). MIR and ED-N are research fellows from CNPq, Brazil.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Coakley, Miss Gillian
Authors: Ramirez, M. I., Amorim, M. G., Gadelha, C., Milic, I., Welsh, J. A., Freitas, V. M., Nawaz, M., Akbar, N., Couch, Y., Makin, L., Cooke, F., Vettore, A. L., Batista, P. X., Freezor, R., Pezuk, J. A., Rosa-Fernandes, L., Carreira, A. C. O., Devitt, A., Jacobs, L., Silva, I. T., Coakley, G., Nunes, D. N., Carter, D., Palmisano, G., and Dias-Neto, E.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Nanoscale
Publisher:Royal Society of Chemistry
ISSN:2040-3364
ISSN (Online):2040-3372
Published Online:13 December 2017

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