Qiu, L., Wang, Y., Wu, H., Sun, Y., Cui, H. , Zhao, W., Yuan, L. and Zhan, C. (2018) Three-dimensional resolution-enhancement divided aperture correlation-differential confocal microscopy with nanometer axial focusing capability. Optics Express, 26(3), pp. 2314-2324. (doi: 10.1364/OE.26.002314) (PMID:29401771)
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Abstract
Divided aperture confocal microscopy (DACM) provides an improved imaging depth, imaging contrast, and working distance at the expense of spatial resolution. Here, we present a new method-divided aperture correlation-differential confocal microscopy (DACDCM) to improve the DACM resolution and the focusing capability, without changing the DACM configuration. DACDCM divides the DACM image spot into two round regions symmetrical about the optical axis. Then the light intensity signals received simultaneously from two round regions by a charge-coupled device (CCD) are processed by correlation manipulation and differential subtraction to improve the DACM spatial resolution and axial focusing capability, respectively. Theoretical analysis and preliminary experiments indicate that, for the excitation wavelength of λ = 632.8 nm, numerical aperture NA = 0.8, and normalized offset vM = 3.2 of the two regions, the DACDCM resolution is improved by 32.5% and 43.1% in the x and z directions, simultaneously, compared with that of the DACM. The axial focusing resolution used for the sample surface profile imaging was also significantly improved to 2 nm.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Cui, Dr Han |
| Authors: | Qiu, L., Wang, Y., Wu, H., Sun, Y., Cui, H., Zhao, W., Yuan, L., and Zhan, C. |
| Subjects: | Q Science > QC Physics |
| College/School: | College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | Optics Express |
| Publisher: | Optical Society of America |
| ISSN: | 1094-4087 |
| ISSN (Online): | 1094-4087 |
| Published Online: | 23 January 2018 |
| Copyright Holders: | Copyright © 2018 Optics Express |
| First Published: | First published in Optics Express 26(3):2314-2324 |
| Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
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