Three-dimensional resolution-enhancement divided aperture correlation-differential confocal microscopy with nanometer axial focusing capability

Qiu, L., Wang, Y., Wu, H., Sun, Y., Cui, H. , Zhao, W., Yuan, L. and Zhan, C. (2018) Three-dimensional resolution-enhancement divided aperture correlation-differential confocal microscopy with nanometer axial focusing capability. Optics Express, 26(3), pp. 2314-2324. (doi:10.1364/OE.26.002314) (PMID:29401771)

Qiu, L., Wang, Y., Wu, H., Sun, Y., Cui, H. , Zhao, W., Yuan, L. and Zhan, C. (2018) Three-dimensional resolution-enhancement divided aperture correlation-differential confocal microscopy with nanometer axial focusing capability. Optics Express, 26(3), pp. 2314-2324. (doi:10.1364/OE.26.002314) (PMID:29401771)

[img]
Preview
Text
154350.pdf - Published Version

4MB

Abstract

Divided aperture confocal microscopy (DACM) provides an improved imaging depth, imaging contrast, and working distance at the expense of spatial resolution. Here, we present a new method-divided aperture correlation-differential confocal microscopy (DACDCM) to improve the DACM resolution and the focusing capability, without changing the DACM configuration. DACDCM divides the DACM image spot into two round regions symmetrical about the optical axis. Then the light intensity signals received simultaneously from two round regions by a charge-coupled device (CCD) are processed by correlation manipulation and differential subtraction to improve the DACM spatial resolution and axial focusing capability, respectively. Theoretical analysis and preliminary experiments indicate that, for the excitation wavelength of λ = 632.8 nm, numerical aperture NA = 0.8, and normalized offset vM = 3.2 of the two regions, the DACDCM resolution is improved by 32.5% and 43.1% in the x and z directions, simultaneously, compared with that of the DACM. The axial focusing resolution used for the sample surface profile imaging was also significantly improved to 2 nm.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cui, Dr Han
Authors: Qiu, L., Wang, Y., Wu, H., Sun, Y., Cui, H., Zhao, W., Yuan, L., and Zhan, C.
Subjects:Q Science > QC Physics
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Optics Express
Publisher:Optical Society of America
ISSN:1094-4087
ISSN (Online):1094-4087
Published Online:23 January 2018
Copyright Holders:Copyright © 2018 Optics Express
First Published:First published in Optics Express 26(3):2314-2324
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

University Staff: Request a correction | Enlighten Editors: Update this record