Mitigating the risk of Zika virus contamination of raw materials and cell lines in the manufacture of biologicals

Zmurko, J., Vasey, D. B., Donald, C. L. , Armstrong, A. A., McKee, M. L., Kohl, A. and Clayton, R. F. (2018) Mitigating the risk of Zika virus contamination of raw materials and cell lines in the manufacture of biologicals. Journal of General Virology, 99, pp. 219-229. (doi:10.1099/jgv.0.000995) (PMID:29239715)

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Abstract

Ensuring the virological safety of biologicals is challenging due to the risk of viral contamination of raw materials and cell banks, and exposure during in-process handling to known and/or emerging viral pathogens. Viruses may contaminate raw materials and biologicals intended for human or veterinary use and remain undetected until appropriate testing measures are employed. The outbreak and expansive spread of the mosquito-borne flavivirus Zika virus (ZIKV) poses challenges to screening human- and animal -derived products used in the manufacture of biologicals. Here, we report the results of an in vitro study where detector cell lines were challenged with African and Asian lineages of ZIKV. We demonstrate that this pathogen is robustly detectable by in vitro assay, thereby providing assurance of detection of ZIKV, and in turn underpinning the robustness of in vitro virology assays in safety testing of biologicals.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Zmurko, Joanna and Donald, Dr Claire and Kohl, Professor Alain
Authors: Zmurko, J., Vasey, D. B., Donald, C. L., Armstrong, A. A., McKee, M. L., Kohl, A., and Clayton, R. F.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of General Virology
Publisher:Microbiology Society
ISSN:0022-1317
ISSN (Online):1465-2099
Published Online:14 December 2017
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Journal of General Virology 99:219-229
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
656341Virus-host interactions in hepatitis C virus infectionJohn McLauchlanMedical Research Council (MRC)MC_UU_12014/1MVLS III - CENTRE FOR VIRUS RESEARCH
708681The emergence of Zika virus in Brazil: investigating prevalence and host responses to design preventive strategiesAlain KohlMedical Research Council (MRC)MR/N017552/1MVLS III - CENTRE FOR VIRUS RESEARCH