Human cytomegalovirus miR-UL112-1 promotes the down-regulation of viral immediate early-gene expression during latency to prevent T-cell recognition of latently infected cells

Lau, B. , Poole, E., Van Damme, E., Bunkens, L., Sowash, M., King, H., Murphy, E., Wills, M., Van Loock, M. and Sinclair, J. (2016) Human cytomegalovirus miR-UL112-1 promotes the down-regulation of viral immediate early-gene expression during latency to prevent T-cell recognition of latently infected cells. Journal of General Virology, 97(9), pp. 2387-2398. (doi: 10.1099/jgv.0.000546) (PMID:27411311)

Abstract

Human cytomegalovirus, a member of the herpesvirus family, can cause significant morbidity and mortality in immune compromised patients resulting from either primary lytic infection or reactivation from latency. Latent infection is associated with a restricted viral transcription programme compared to lytic infection which consists of defined protein coding RNAs but also includes a number of virally encoded microRNAs (miRNAs). One of these, miR-UL112-1, is known to target the major lytic IE72 transcript but, to date, a functional role for miR-UL112-1 during latent infection has not been shown. To address this, we have analysed latent infection in myeloid cells using a virus in which the target site for miR-UL112-1 in the 3′ UTR of IE72 was removed such that any IE72 RNA present during latent infection would no longer be subject to regulation by miR-UL112-1 through the RNAi pathway. Our data show that removal of the miR-UL112-1 target site in IE72 results in increased levels of IE72 RNA in experimentally latent primary monocytes. Furthermore, this resulted in induction of immediate early (IE) gene expression that is detectable by IE-specific cytotoxic T-cells (CTLs); no such CTL recognition of monocytes latently infected with wild-type virus was observed. We also recapitulated these findings in the more tractable THP-1 cell line model of latency. These observations argue that an important role for miR-UL112-1 during latency is to ensure tight control of lytic viral immediate early (IE) gene expression thereby preventing recognition of latently infected cells by the host's potent pre-existing anti-viral CTL response.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Lau, Dr Betty
• Authors: Lau, B., Poole, E., Van Damme, E., Bunkens, L., Sowash, M., King, H., Murphy, E., Wills, M., Van Loock, M., and Sinclair, J.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Journal of General Virology
• Publisher: Microbiology Society
• ISSN: 0022-1317
• ISSN (Online): 1465-2099
• Published Online: 01 September 2016
• Copyright Holders: Copyright © 2016 The Authors
• First Published: First published in Journal of General Virology 97(9):2387-2398
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher