Superchiral plasmonic phase sensitivity for fingerprinting of protein interface structure

Tullius, R. et al. (2017) Superchiral plasmonic phase sensitivity for fingerprinting of protein interface structure. ACS Nano, 11(12), pp. 12049-12056. (doi:10.1021/acsnano.7b04698) (PMID:29220155)

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Abstract

The structure adopted by biomaterials, such as proteins, at interfaces is a crucial parameter in a range of important biological problems. It is a critical property in defining the functionality of cell/bacterial membranes and biofilms (i.e., in antibiotic-resistant infections) and the exploitation of immobilized enzymes in biocatalysis. The intrinsically small quantities of materials at interfaces precludes the application of conventional spectroscopic phenomena routinely used for (bio)structural analysis due to a lack of sensitivity. We show that the interaction of proteins with superchiral fields induces asymmetric changes in retardation phase effects of excited bright and dark modes of a chiral plasmonic nanostructure. Phase retardations are obtained by a simple procedure, which involves fitting the line shape of resonances in the reflectance spectra. These interference effects provide fingerprints that are an incisive probe of the structure of interfacial biomolecules. Using these fingerprints, layers composed of structurally related proteins with differing geometries can be discriminated. Thus, we demonstrate a powerful tool for the bioanalytical toolbox.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:TULLIUS, Ryan and Gadegaard, Professor Nikolaj and Karimullah, Dr Affar and Cooke, Professor Graeme and Barron, Professor Laurence and Lapthorn, Dr Adrian and Kadodwala, Professor Malcolm
Authors: Tullius, R., Platt, G. W., Khosravi Khorashad, L., Gadegaard, N., Lapthorn, A. J., Rotello, V. M., Cooke, G., Barron, L. D., Govorov, A. O., Karimullah, A. S., and Kadodwala, M.
College/School:College of Science and Engineering > School of Chemistry
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:ACS Nano
Publisher:American Chemical Society
ISSN:1936-0851
ISSN (Online):1936-086X
Published Online:08 December 2017
Copyright Holders:Copyright © 2017 American Chemical Society
First Published:First published in ACS Nano 11(12): 12049-12056
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
618701Plasmon-Enhanced Chiroptical SensorsMalcolm KadodwalaEngineering and Physical Sciences Research Council (EPSRC)EP/K034936/1CHEM - CHEMISTRY
726421Mapping the mesoscale structural landscape using "sculpted" chiral plasmonic fieldsMalcolm KadodwalaEngineering and Physical Sciences Research Council (EPSRC)EP/P00086X/1CHEM - CHEMISTRY
683531Consortium for advanced materials based on spin chiralityRobert StampsEngineering and Physical Sciences Research Council (EPSRC)EP/M024423/1S&E P&A - PHYSICS & ASTRONOMY