Construction of a 5.2-megabase physical map of the human X chromosome at Xq22 using pulsed-field gel electrophoresis and yeast artificial chromosomes

Vetrie, D. , Bobrow, M. and Harris, A. (1993) Construction of a 5.2-megabase physical map of the human X chromosome at Xq22 using pulsed-field gel electrophoresis and yeast artificial chromosomes. Genomics, 15(3), pp. 631-642. (doi:10.1006/geno.1993.1118) (PMID:8096829)

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Abstract

Several genes involved in human genetic diseases map to the Xq22 band on the long arm of the human X chromosome. We have constructed a long-range restriction map of the most proximal part of Xq22. Initially, pulsed-field gel electrophoresis, in combination with rare-cutting restriction enzymes, was used to try and establish physical linkage of 11 polymorphic and non-polymorphic DNA markers. This approach resulted in the construction of three long-range restriction maps around groups of physically linked Xq22 markers that spanned over 5.0 Mb of DNA. Yeast artificial chromosome clones were used to organize the three long-range maps onto a contiguous 5.2-Mb stretch of Xq22. The order of markers in this region was shown to be cen-GLA-DXS178-DXS101-DXS83-DXS24-DXS101-DXS54-PLP-DXS94-DXS147-DXS17-DXS87-tel. The results of this study suggest that the proximal part of Xq22 may be rich in genes. Construction of a physical map for this region will, therefore, facilitate the localization and subsequent isolation of novel genes.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Vetrie, Dr David
Authors: Vetrie, D., Bobrow, M., and Harris, A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Genomics
Publisher:Elsevier
ISSN:0888-7543
ISSN (Online):1089-8646

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