Physical mapping shows close linkage between the α-galactosidase A gene (GLA) and the DXS178 locus

Vetrie, D. , Bentley, D., Bobrow, M. and Harris, A. (1993) Physical mapping shows close linkage between the α-galactosidase A gene (GLA) and the DXS178 locus. Human Genetics, 92(1), pp. 95-99. (doi:10.1007/BF00216154) (PMID:8103505)

Full text not currently available from Enlighten.

Abstract

X-linked agammaglobulinaemia (XLA) is an inherited disorder characterised by a lack of circulating B-cells and antibodies. While the gene involved in XLA has not yet been identified, the locus for the disorder is tightly linked to the polymorphic marker DXS178, which maps to Xq22. Fabry disease is an X-linked recessive disorder caused by a deficiency in the lysosomal enzyme α-galactosidase A. The gene encoding this enzyme has been characterized and also maps to Xq22. Using pulsed field gel electrophoresis we have constructed a long-range restriction map that shows that the α-galactosidase A gene (GLA) and DXS178 lie no more than 140 kb apart on a stretch of DNA containing a number of putative CpG islands. We have also isolated yeast artifical chromosome (YAC) clones that confirm this physical linkage. The localisation of DXS178 near the α-galactosidase A gene will facilitate carrier detection in Fabry families using restriction fragment length polymorphism (RFLP) analysis. The identification of a number of CpG islands near DXS178 also provides candidate locations for the gene responsible for XLA.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Vetrie, Dr David
Authors: Vetrie, D., Bentley, D., Bobrow, M., and Harris, A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Human Genetics
Publisher:Springer
ISSN:0340-6717
ISSN (Online):1432-1203

University Staff: Request a correction | Enlighten Editors: Update this record