A global characterisation of the archaeal transcription machinery

Smollett, K. , Blombach, F., Fouqueau, T. and Werner, F. (2017) A global characterisation of the archaeal transcription machinery. In: Clouet-d'Orval, B. (ed.) RNA Metabolism and Gene Expression in Archaea. Series: Nucleic acids and molecular biology, 32 (32). Springer International Publishing, pp. 1-26. ISBN 9783319657943 (doi: 10.1007/978-3-319-65795-0_1)

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Publisher's URL: https://link.springer.com/book/10.1007/978-3-319-65795-0


Archaea employ a eukaryote-like transcription apparatus to transcribe a bacteria-like genome; while the RNA polymerase, basal factors and promoter elements mirror the eukaryotic RNA polymerase II system, archaeal genomes are densely packed with genes organised into multicistronic transcription units. The molecular mechanisms of archaeal transcription have been studied and characterised in great detail in vitro, but until recently relatively little was known about its global characteristics. In this chapter we discuss an integrated view of transcription from the molecular to the global level. Systems biology approaches have provided compelling insights into promoter and terminator DNA elements, the genome-wide distribution of transcription initiation- and elongation factors and RNA polymerase, the archaeal transcriptome and chromatin organisation. Overall these analyses illuminate transcription from a genome-wide perspective and serve as a resource for the community. In addition, Big Data can often validate mechanistic models based on biochemical and structural information, and generate new working hypotheses that can be thoroughly tested and dissected in vitro. This is an exciting time to study gene expression in the archaea since we are at the brink of a comprehensive yet detailed understanding of transcription.

Item Type:Book Sections
Glasgow Author(s) Enlighten ID:Smollett, Dr Katherine
Authors: Smollett, K., Blombach, F., Fouqueau, T., and Werner, F.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
Publisher:Springer International Publishing
Published Online:31 October 2017

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