Watson, E., Sherry, A. , Inglis, N. F., Lainson, A., Jyothi, D., Yaga, R., Manson, E., Imrie, L., Everest, P. and Smith, D. G.E. (2014) Proteomic and genomic analysis reveals novel Campylobacter jejuni outer membrane proteins and potential heterogeneity. EuPA Open Proteomics, 4, pp. 184-194. (doi: 10.1016/j.euprot.2014.06.003) (PMID:27525220) (PMCID:PMC4975774)
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Abstract
Gram negative bacterial outer membrane proteins play important roles in the interaction of bacteria with their environment including nutrient acquisition, adhesion and invasion, and antibiotic resistance. In this study we identified 47 proteins within the Sarkosyl-insoluble fraction of Campylobacter jejuni81-176, usingLC–ESI-MS/MS. Comparative analysis of outer membrane protein sequences was visualised to reveal protein distribution within a panel of Campylobacter spp., identifying several C.jejuni specific proteins. Smith–Waterman analyses of C.jejuni homologues revealed high sequence conservation amongst a number of hypothetical proteins, sequence heterogeneity of other proteins and several proteins which are absent in a proportion of strains.
| Item Type: | Articles (Other) |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Everest, Dr Paul and Smith, Professor David and Kerr, Mrs Erin and Linn, Dr Aileen |
| Authors: | Watson, E., Sherry, A., Inglis, N. F., Lainson, A., Jyothi, D., Yaga, R., Manson, E., Imrie, L., Everest, P., and Smith, D. G.E. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | EuPA Open Proteomics |
| Publisher: | Elsevier |
| ISSN: | 2212-9685 |
| ISSN (Online): | 2212-9685 |
| Published Online: | 26 June 2014 |
| Copyright Holders: | Copyright © 2014 The Author |
| First Published: | First published in EuPA Open Proteomics 4:184-194 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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