The M25 gene products are critical for the cytopathic effect of mouse cytomegalovirus

Kutle, I. et al. (2017) The M25 gene products are critical for the cytopathic effect of mouse cytomegalovirus. Scientific Reports, 7, 15588. (doi:10.1038/s41598-017-15783-x) (PMID:29138436) (PMCID:PMC5686157)

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Abstract

Cell rounding is a hallmark of the cytopathic effect induced by cytomegaloviruses. By screening a panel of deletion mutants of mouse cytomegalovirus (MCMV) a mutant was identified that did not elicit cell rounding and lacked the ability to form typical plaques. Altered cell morphology was assigned to the viral M25 gene. We detected an early 2.8 kb M25 mRNA directing the synthesis of a 105 kDa M25 protein, and confirmed that a late 3.1 kb mRNA encodes a 130 kDa M25 tegument protein. Virions lacking the M25 tegument protein were of smaller size because the tegument layer between capsid and viral envelope was reduced. The ΔM25 mutant did not provoke the rearrangement of the actin cytoskeleton observed after wild-type MCMV infection, and isolated expression of the M25 proteins led to cell size reduction, confirming that they contribute to the morphological changes. Yields of progeny virus and cell-to-cell spread of the ΔM25 mutant in vitro were diminished and replication in vivo was impaired. The identification of an MCMV gene involved in cell rounding provides the basis for investigating the role of this cytopathic effect in CMV pathogenesis.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Glass, Dr Mandy
Authors: Kutle, I., Sengstake, S., Templin, C., Glaß, M., Kubsch, T., Keyser, K. A., Binz, A., Bauerfeind, R., Sodeik, B., Čičin-Šain, L., Dezeljin, M., and Messerle, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Scientific Reports
Publisher:Nature Publishing Group
ISSN:2045-2322
ISSN (Online):2045-2322
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Scientific Reports 7: 15588
Publisher Policy:Reproduced under a Creative Commons License

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