A real-time monitoring and detection instrument for analysis of the effects of O3 on bioluminescent Escherichia coli on agar surfaces - potential applications to the food industry

Tan, B. K., Watson, I. A. , Parton, R. and Peden, I. (2005) A real-time monitoring and detection instrument for analysis of the effects of O3 on bioluminescent Escherichia coli on agar surfaces - potential applications to the food industry. Innovative Food Science and Emerging Technologies, 6(2), pp. 183-188. (doi: 10.1016/j.ifset.2004.12.003)

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Abstract

An instrument was fabricated to measure the real-time response of a bioluminescent construct of <i>Escherichia coli</i> to ozone treatment. The bioluminescent output from <i>E. Coli</i> inoculated on nutrient agar plates was measured with a photomultiplier tube coupled to a lock-in amplifier and the ozone concentration was calculated from absorption measurements from a 254 nm source. Data from the two measurement systems were acquired in real-time via an oscilloscope, captured to a PC for analysis and displayed on the graphical user interface. The ozone and bioluminescence measurements were done in two different chambers to isolate the measurement systems and to prevent any adverse effects of the UV radiation on the bacteria. The real-time data showed the instantaneous changes in the bioluminescence output with increasing ozone concentration. The dark current from plastic petri dishes both with and without agar was measured and a peak to peak variation of 0.10 dB in the signal was observed; this was negligible compared to the bioluminescent signal.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Watson, Dr Ian and Peden, Mr Ian and Parton, Dr Roger
Authors: Tan, B. K., Watson, I. A., Parton, R., and Peden, I.
College/School:College of Science and Engineering > School of Engineering > Systems Power and Energy
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Innovative Food Science and Emerging Technologies
Publisher:Elsevier Ltd.
ISSN:1466-8564
ISSN (Online):1878-5522
Published Online:07 April 2005

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