Spectrally selective fluorescence imaging of Chlorobaculum tepidum reaction centers conjugated to chelator-modified silver nanowires

Kowalska, D., Szalkowski, M., Ashraf, K., Grzelak, J., Lokstein, H., Niedziolka-Jonsson, J., Cogdell, R. and Mackowski, S. (2018) Spectrally selective fluorescence imaging of Chlorobaculum tepidum reaction centers conjugated to chelator-modified silver nanowires. Photosynthesis Research, 135(1-3), pp. 329-336. (doi: 10.1007/s11120-017-0455-y) (PMID:29090426)

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Abstract

A polyhistidine tag (His-tag) present on Chlorobaculum tepidum reaction centers (RCs) was used to immobilize photosynthetic complexes on a silver nanowire (AgNW) modified with nickel-chelating nitrilo-triacetic acid (Ni-NTA). The optical properties of conjugated nanostructures were studied using wide-field and confocal fluorescence microscopy. Plasmonic enhancement of RCs conjugated to AgNWs was observed as their fluorescence intensity dependence on the excitation wavelength does not follow the excitation spectrum of RC complexes in solution. The strongest effect of plasmonic interactions on the emission intensity of RCs coincides with the absorption spectrum of AgNWs and is observed for excitation into the carotenoid absorption. From the absence of fluorescence decay shortening, we attribute the emission enhancement to increase of absorption in RC complexes.

Item Type:Articles
Keywords:Bacteriochlorophyll, conjugation, fluorescence, plasmonic enhancement, silver nanowires.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Lokstein, Dr Rer Nat Heiko and Cogdell, Professor Richard and Ashraf, Mr Khuram
Authors: Kowalska, D., Szalkowski, M., Ashraf, K., Grzelak, J., Lokstein, H., Niedziolka-Jonsson, J., Cogdell, R., and Mackowski, S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Photosynthesis Research
Publisher:Springer
ISSN:0166-8595
ISSN (Online):1573-5079
Published Online:31 October 2017
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Photosynthesis Research 135(1-3): 329-336
Publisher Policy:Reproduced under a Creative Commons License

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