Devaney, E. (1981) The development of Dirofilaria immitis in cultured malpighian tubules. Acta Tropica, 38(3), pp. 251-60. (PMID:6118033)
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Abstract
A technique is described for the cultivation in vitro of Dirofilaria immitis to the second larval stage within malpighian tubules excised from mosquitoes 20-24 h post infection. The malpighian tubules were removed from mosquitoes under aseptic conditions, washed and inoculated into a variety of tissue culture media, both insect and mammalian. In some cultures a feeder layer of Aedes malayensis cells was included. The 24 h larvae developed to the second stage only in Schneider's Drosophila medium containing 20% foetal bovine serum, irrespective of whether a cell layer was included in the cultures. Development of the larvae to the infective third stage was not observed.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Devaney, Professor Eileen |
| Authors: | Devaney, E. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | Acta Tropica |
| Publisher: | Elsevier |
| ISSN: | 0001-706X |
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