Dirofilaria immitis: the moulting of the infective larva in vitro.

Devaney, E. (1985) Dirofilaria immitis: the moulting of the infective larva in vitro. Journal of Helminthology, 59(1), pp. 47-50. (PMID:3989262)

Full text not currently available from Enlighten.

Abstract

A number of in vitro culture systems were tested for their ability to support the development of Dirofilaria immitis infective larvae to the fourth larval stage. In cultures of medium ML-15 containing a feeder layer of Dog Sarcoma (DS) cells larvae successfully moulted and showed a small but significant increase in length. Ultrastructural observations demonstrated that the fourth-stage cuticle was synthesized in vitro and in some larvae was fully formed by 60 hours of culture. The hypodermis of moulting larvae contained numerous multi-vesicular bodies. It is concluded that the moult in vitro is a true moult and not an atypical response of the larvae to the conditions of culture.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Devaney, Professor Eileen
Authors: Devaney, E.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Journal of Helminthology
Publisher:Cambridge University Press
ISSN:0022-149X
ISSN (Online):1475-2697

University Staff: Request a correction | Enlighten Editors: Update this record