The analysis of the humoral response of the BALB/c mouse immunized with radiation attenuated third stage larvae of Brugia pahangi

Bancroft, A. and Devaney, E. (1993) The analysis of the humoral response of the BALB/c mouse immunized with radiation attenuated third stage larvae of Brugia pahangi. Parasite Immunology, 15(3), pp. 153-162. (doi:10.1111/j.1365-3024.1993.tb00595.x) (PMID:8316409)

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Abstract

BALB/c mice immunized with L3 of Brugia pahangi, irradiated at 45kRad from a 137Caesium source, are strongly immune to challenge infection (75–100% reduction in the recovery of challenge infection larvae on day 6 post-challenge). The target of immunity appears to be the post-infective L3, as challenge infection larvae are killed within 5–6 days of infection. By immunoblot analysis, serum from immune animals recognizes a limited set of somatic antigens, the majority of which are shared between different life cycle stages. Serum from immune mice also strongly recognizes larval surface antigens by immunofluorescence, some of which may be stage specific. The larval surface determinants do not appear to be protein or glycoprotein by standard immunochemical analysis. A proportion of the antibody response of the BALB/c mouse is directed towards phosphorylcholine epitopes on filarial antigens, but the limited antigen recognition cannot be explained on the basis of the mouse strain used, as CBA/Ca mice recognize a similar limited set of antigens.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Devaney, Professor Eileen
Authors: Bancroft, A., and Devaney, E.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Parasite Immunology
Publisher:Wiley
ISSN:0141-9838
ISSN (Online):1365-3024

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