Particle separation by phase modulated surface acoustic waves

Simon, G., Andrade, M. A.B., Reboud, J. , Marques-Hueso, J., Desmulliez, M. P.Y., Cooper, J. M. , Riehle, M. O. and Bernassau, A. L. (2017) Particle separation by phase modulated surface acoustic waves. Biomicrofluidics, 11(5), 054115. (doi:10.1063/1.5001998)

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Abstract

High efficiency isolation of cells or particles from a heterogeneous mixture is a critical processing step in lab-on-a-chip devices. Acoustic techniques offer contactless and label-free manipulation, preserve viability of biological cells, and provide versatility as the applied electrical signal can be adapted to various scenarios. Conventional acoustic separation methods use time-of-flight and achieve separation up to distances of quarter wavelength with limited separation power due to slow gradients in the force. The method proposed here allows separation by half of the wavelength and can be extended by repeating the modulation pattern and can ensure maximum force acting on the particles. In this work, we propose an optimised phase modulation scheme for particle separation in a surface acoustic wave microfluidic device. An expression for the acoustic radiation force arising from the interaction between acoustic waves in the fluid was derived. We demonstrated, for the first time, that the expression of the acoustic radiation force differs in surface acoustic wave and bulk devices, due to the presence of a geometric scaling factor. Two phase modulation schemes are investigated theoretically and experimentally. Theoretical findings were experimentally validated for different mixtures of polystyrene particles confirming that the method offers high selectivity. A Monte-Carlo simulation enabled us to assess performance in real situations, including the effects of particle size variation and non-uniform acoustic field on sorting efficiency and purity, validating the ability to separate particles with high purity and high resolution.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Riehle, Dr Mathis and Cooper, Professor Jonathan and Reboud, Dr Julien
Authors: Simon, G., Andrade, M. A.B., Reboud, J., Marques-Hueso, J., Desmulliez, M. P.Y., Cooper, J. M., Riehle, M. O., and Bernassau, A. L.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Biomicrofluidics
Publisher:AIP Publishing
ISSN:1932-1058
ISSN (Online):1932-1058
Published Online:26 October 2017
Copyright Holders:Copyright © 2017 AIP Publishing
First Published:First published in Biomicrofluidics 11(5): 054115
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
617021Advanced Diagnostics using PhononicsJonathan CooperEngineering and Physical Sciences Research Council (EPSRC)EP/K027611/1ENG - BIOMEDICAL ENGINEERING