Tracking elusive cargo: Illuminating spatio-temporal type 3 effector protein dynamics using reporters

O'Boyle, N., Connolly, J. P.R. and Roe, A. J. (2018) Tracking elusive cargo: Illuminating spatio-temporal type 3 effector protein dynamics using reporters. Cellular Microbiology, 20(1), e12797. (doi: 10.1111/cmi.12797) (PMID:29087624) (PMCID:PMC5765406)

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Abstract

Type 3 secretion systems (T3SS) form an integral part of the arsenal of many pathogenic bacteria. These injection machines, together with their cargo of subversive effector proteins are capable of manipulating the cellular environment of the host in order to ensure persistence of the pathogen. In order to fully appreciate the functions of Type 3 effectors it is necessary to gain spatio-temporal knowledge of each effector during the process of infection. A number of genetic modifications have been exploited in order to reveal effector protein secretion, translocation and subsequent activity and localisation within host cells. In this review, we will discuss the many available approaches for tracking effector protein dynamics and discuss the challenges faced to improve the current technologies and gain a clearer picture of effector protein function.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Connolly, Dr James and Roe, Professor Andrew and O'Boyle, Dr Nicky
Authors: O'Boyle, N., Connolly, J. P.R., and Roe, A. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Cellular Microbiology
Publisher:Wiley-Blackwell Publishing
ISSN:1462-5814
ISSN (Online):1462-5822
Published Online:31 October 2017
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Cellular Microbiology 20(1): e12797
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
751711The role of the host metabolite D-serine in development of neonatal meningitisAndrew RoeGlasgow Children's Hospital Charity (GCHC)GCHC/PSG/2016/02 RoeIII - BACTERIOLOGY
691101Where and Why: The Influence of Host Metabolism on Bacterial Niche SpecificityAndrew RoeBiotechnology and Biological Sciences Research Council (BBSRC)BB/M029646/1III - BACTERIOLOGY