O'Boyle, N., Connolly, J. P.R. and Roe, A. J. (2018) Tracking elusive cargo: Illuminating spatio-temporal type 3 effector protein dynamics using reporters. Cellular Microbiology, 20(1), e12797. (doi: 10.1111/cmi.12797) (PMID:29087624) (PMCID:PMC5765406)
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Abstract
Type 3 secretion systems (T3SS) form an integral part of the arsenal of many pathogenic bacteria. These injection machines, together with their cargo of subversive effector proteins are capable of manipulating the cellular environment of the host in order to ensure persistence of the pathogen. In order to fully appreciate the functions of Type 3 effectors it is necessary to gain spatio-temporal knowledge of each effector during the process of infection. A number of genetic modifications have been exploited in order to reveal effector protein secretion, translocation and subsequent activity and localisation within host cells. In this review, we will discuss the many available approaches for tracking effector protein dynamics and discuss the challenges faced to improve the current technologies and gain a clearer picture of effector protein function.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Roe, Professor Andrew and O Boyle, Dr Nicky and Connolly, Dr James |
| Authors: | O'Boyle, N., Connolly, J. P.R., and Roe, A. J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Cellular Microbiology |
| Publisher: | Wiley-Blackwell Publishing |
| ISSN: | 1462-5814 |
| ISSN (Online): | 1462-5822 |
| Published Online: | 31 October 2017 |
| Copyright Holders: | Copyright © 2017 The Authors |
| First Published: | First published in Cellular Microbiology 20(1): e12797 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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