Repression of RNA polymerase by the archaeo-viral regulator ORF145/RIP

Sheppard, C. et al. (2016) Repression of RNA polymerase by the archaeo-viral regulator ORF145/RIP. Nature Communications, 7, 13595. (doi: 10.1038/ncomms13595) (PMID:27882920) (PMCID:PMC5123050)

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Little is known about how archaeal viruses perturb the transcription machinery of their hosts. Here we provide the first example of an archaeo-viral transcription factor that directly targets the host RNA polymerase (RNAP) and efficiently represses its activity. ORF145 from the temperate Acidianus two-tailed virus (ATV) forms a high-affinity complex with RNAP by binding inside the DNA-binding channel where it locks the flexible RNAP clamp in one position. This counteracts the formation of transcription pre-initiation complexes in vitro and represses abortive and productive transcription initiation, as well as elongation. Both host and viral promoters are subjected to ORF145 repression. Thus, ORF145 has the properties of a global transcription repressor and its overexpression is toxic for Sulfolobus. On the basis of its properties, we have re-named ORF145 RNAP Inhibitory Protein (RIP).

Item Type:Articles
Glasgow Author(s) Enlighten ID:Smollett, Dr Katherine
Authors: Sheppard, C., Blombach, F., Belsom, A., Schulz, S., Daviter, T., Smollett, K., Mahieu, E., Erdmann, S., Tinnefeld, P., Garrett, R., Grohmann, D., Rappsilber, J., and Werner, F.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Nature Communications
Publisher:Nature Publishing Group
ISSN (Online):2041-1723
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in Nature Communications 7:13595
Publisher Policy:Reproduced under a Creative Commons License

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