A time-course study of circulating antigen and parasite-specific antibody in cotton rats infected with Leishmania donovani

Azazy, A. A., Chance, M. L. and Devaney, E. (1997) A time-course study of circulating antigen and parasite-specific antibody in cotton rats infected with Leishmania donovani. Annals of Tropical Medicine and Parasitology, 91(2), pp. 153-162. (doi:10.1080/00034983.1997.11813125) (PMID:9307657)

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Abstract

Levels of circulating antigen in a group of cotton rats infected with Leishmania donovani were followed over a 28-week period, using a modified, polyethylene-glycol (PEG) ELISA. Circulating antigen could be detected from 1 week post-infection and gradually increased over time. In infected cotton rats treated with a curative dose of Pentostam at 12 weeks post-infection, antigen levels peaked and then declined. Antigen was still detected in some of the treated animals at 28 weeks post-infection. The antibodies used in the PEG-ELISA were also used in a capture ELISA to detect parasite antigens in urine. Urine samples which were positive by capture ELISA were also analysed by western blotting, in an attempt to identify the parasite antigens present. Three components, of 45, 47 and 58 kDa, were detected.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Devaney, Professor Eileen
Authors: Azazy, A. A., Chance, M. L., and Devaney, E.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Annals of Tropical Medicine and Parasitology
Publisher:Taylor & Francis
ISSN:0003-4983
ISSN (Online):1364-8594
Published Online:15 November 2016

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