Conservation of a microRNA cluster in parasitic nematodes and profiling of miRNAs in excretory-secretory products and microvesicles of Haemonchus contortus

Gu, H. Y., Marks, N. D., Winter, A. D., Weir, W. , Tzelos, T., McNeilly, T. N., Britton, C. and Devaney, E. (2017) Conservation of a microRNA cluster in parasitic nematodes and profiling of miRNAs in excretory-secretory products and microvesicles of Haemonchus contortus. PLoS Neglected Tropical Diseases, 11(11), e0006056. (doi:10.1371/journal.pntd.0006056) (PMID:29145392) (PMCID:PMC5709059)

Gu, H. Y., Marks, N. D., Winter, A. D., Weir, W. , Tzelos, T., McNeilly, T. N., Britton, C. and Devaney, E. (2017) Conservation of a microRNA cluster in parasitic nematodes and profiling of miRNAs in excretory-secretory products and microvesicles of Haemonchus contortus. PLoS Neglected Tropical Diseases, 11(11), e0006056. (doi:10.1371/journal.pntd.0006056) (PMID:29145392) (PMCID:PMC5709059)

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Abstract

microRNAs are small non-coding RNAs that are important regulators of gene expression in a range of animals, including nematodes. We have analysed a cluster of four miRNAs from the pathogenic nematode species Haemonchus contortus that are closely linked in the genome. We find that the cluster is conserved only in clade V parasitic nematodes and in some ascarids, but not in other clade III species nor in clade V free-living nematodes. Members of the cluster are present in parasite excretory-secretory products and can be detected in the abomasum and draining lymph nodes of infected sheep, indicating their release in vitro and in vivo. As observed for other parasitic nematodes, H. contortus adult worms release extracellular vesicles (EV). Small RNA libraries were prepared from vesicle-enriched and vesicle-depleted supernatants from both adult worms and L4 stage larvae. Comparison of the miRNA species in the different fractions indicated that specific miRNAs are packaged within vesicles, while others are more abundant in vesicle-depleted supernatant. Hierarchical clustering analysis indicated that the gut is the likely source of vesicle-associated miRNAs in the L4 stage, but not in the adult worm. These findings add to the growing body of work demonstrating that miRNAs released from parasitic helminths may play an important role in host-parasite interactions.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Devaney, Professor Eileen and Weir, Dr William and Mcneilly, Dr Tom and Britton, Dr Collette and Winter, Dr Alan
Authors: Gu, H. Y., Marks, N. D., Winter, A. D., Weir, W., Tzelos, T., McNeilly, T. N., Britton, C., and Devaney, E.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:PLoS Neglected Tropical Diseases
Publisher:Public Library of Science
ISSN:1935-2727
ISSN (Online):1935-2735
Published Online:16 November 2017
Copyright Holders:Copyright © 2017 Gu et al.
First Published:First published in PLoS Neglected Tropical Diseases 11(11):e0006056
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
491471Comparative genomics of nematodes: defining the role of miRNAs in developmentEileen DevaneyWellcome Trust (WELLCOTR)086823III - PARASITOLOGY