FlyAtlas 2: a new version of the Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

Leader, D. P. , Krause, S. A. , Pandit, A. , Davies, S. A. and Dow, J. A.T. (2017) FlyAtlas 2: a new version of the Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data. Nucleic Acids Research, 46(D1), D809-D815. (doi:10.1093/nar/gkx976) (PMID:29069479) (PMCID:PMC5753349)

Leader, D. P. , Krause, S. A. , Pandit, A. , Davies, S. A. and Dow, J. A.T. (2017) FlyAtlas 2: a new version of the Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data. Nucleic Acids Research, 46(D1), D809-D815. (doi:10.1093/nar/gkx976) (PMID:29069479) (PMCID:PMC5753349)

[img]
Preview
Text
149992.pdf - Published Version
Available under License Creative Commons Attribution.

1MB

Abstract

FlyAtlas 2 (www.flyatlas2.org) is part successor, part complement to the FlyAtlas database and web application for studying the expression of the genes of Drosophila melanogaster in different tissues of adults and larvae. Although generated in the same lab with the same fly line raised on the same diet as FlyAtlas, the FlyAtlas2 resource employs a completely new set of expression data based on RNA-Seq, rather than microarray analysis, and so it allows the user to obtain information for the expression of different transcripts of a gene. Furthermore, the data for somatic tissues are now available for both male and female adult flies, allowing studies of sexual dimorphism. Gene coverage has been extended by the inclusion of microRNAs and many of the RNA genes included in Release 6 of the Drosophila reference genome. The web interface has been modified to accommodate the extra data, but at the same time has been adapted for viewing on small mobile devices. Users also have access to the RNA-Seq reads displayed alongside the annotated Drosophila genome in the (external) UCSC browser, and are able to link out to the previous FlyAtlas resource to compare the data obtained by RNA-Seq with that obtained using microarrays.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Pandit, Mr Aniruddha and Leader, Dr David and Krause, Dr Sue and Dow, Professor Julian and Davies, Professor Shireen
Authors: Leader, D. P., Krause, S. A., Pandit, A., Davies, S. A., and Dow, J. A.T.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Nucleic Acids Research
Publisher:Oxford University Press
ISSN:0305-1048
ISSN (Online):1362-4962
Published Online:24 October 2017
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Nucleic Acids Research 46(D1):D809-D815
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
609661FlyAtlas2: a definitive, expanded NGS expression resource for the Drosophila community and beyond.Julian DowBiotechnology and Biological Sciences Research Council (BBSRC)BB/K019953/1RI MOLECULAR CELL & SYSTEMS BIOLOGY