Microfluidics-based approaches to the isolation of African trypanosomes

Barrett, M. P. , Cooper, J. M. , Regnault, C., Holm, S. H., Beech, J. P., Tegenfeldt, J. O. and Hochstetter, A. (2017) Microfluidics-based approaches to the isolation of African trypanosomes. Pathogens, 6(4), 47. (doi:10.3390/pathogens6040047) (PMID:28981471) (PMCID:PMC5750571)

[img]
Preview
Text
149914.pdf - Published Version
Available under License Creative Commons Attribution.

4MB

Abstract

African trypanosomes are responsible for significant levels of disease in both humans and animals. The protozoan parasites are free-living flagellates, usually transmitted by arthropod vectors, including the tsetse fly. In the mammalian host they live in the bloodstream and, in the case of human-infectious species, later invade the central nervous system. Diagnosis of the disease requires the positive identification of parasites in the bloodstream. This can be particularly challenging where parasite numbers are low, as is often the case in peripheral blood. Enriching parasites from body fluids is an important part of the diagnostic pathway. As more is learned about the physicochemical properties of trypanosomes, this information can be exploited through use of different microfluidic-based approaches to isolate the parasites from blood or other fluids. Here, we discuss recent advances in the use of microfluidics to separate trypanosomes from blood and to isolate single trypanosomes for analyses including drug screening.

Item Type:Articles
Keywords:DLD, concentration ramping, diagnosis, dielectrophoresis, drug testing, isolation, microfluidics, optical trap, separation, trypanosomes.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Regnault, Mr Clement and Cooper, Professor Jonathan and Barrett, Professor Michael and Hochstetter, Dr Axel
Authors: Barrett, M. P., Cooper, J. M., Regnault, C., Holm, S. H., Beech, J. P., Tegenfeldt, J. O., and Hochstetter, A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Pathogens
Publisher:MDPI
ISSN:2076-0817
ISSN (Online):2076-0817
Published Online:05 October 2017
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Pathogens 6(4): 47
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
371799The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOTR)104111/Z/14/Z & AIII - PARASITOLOGY