Off-axis electron holography of bacterial cells and magnetic nanoparticles in liquid

Prozorov, T., Almeida, T. P. , Kovács, A. and Dunin-Borkowski, R. E. (2017) Off-axis electron holography of bacterial cells and magnetic nanoparticles in liquid. Journal of the Royal Society: Interface, 14(135), 20170464. (doi:10.1098/rsif.2017.0464) (PMID:29021160)

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Abstract

The mapping of electrostatic potentials and magnetic fields in liquids usingelectron holography has been considered to be unrealistic. Here, we showthat hydrated cells ofMagnetospirillum magneticumstrain AMB-1 and assem-blies of magnetic nanoparticles can be studied using off-axis electronholography in a fluid cell specimen holder within the transmission electronmicroscope. Considering that the holographic object and reference waveboth pass through liquid, the recorded electron holograms show sufficientinterference fringe contrast to permit reconstruction of the phase shift ofthe electron wave and mapping of the magnetic induction from bacterialmagnetite nanocrystals. We assess the challenges of performingin situmagne-tization reversal experiments using a fluid cell specimen holder, discussapproaches for improving spatial resolution and specimen stability, and outlinefuture perspectives for studying scientific phenomena, ranging from interpar-ticle interactions in liquids and electrical double layers at solid–liquidinterfaces to biomineralization and the mapping of electrostatic potentialsassociated with protein aggregation and folding.

Item Type:Articles (Letter)
Additional Information:The present research on in situ electron microscopy with a fluid cell was supported by the US Department of Energy, Office of Science, Basic Energy Sciences, Materials Sciences and Engineering Division. T.P. acknowledges support from a Department of Energy Office of Science Early Career Research Award, Biomolecular Materials Program. The work at Ames Laboratory was supported by the US Department of Energy, Office of Science, Basic Energy Sciences, Materials Science and Engineering Division. The Ames Laboratory is operated for the US Department of Energy by Iowa State University under contract # DE-AC02-07CH11358. The research leading to these results received funding from the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013)/ ERC grant agreement no. 320832.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Almeida, Dr Trevor
Authors: Prozorov, T., Almeida, T. P., Kovács, A., and Dunin-Borkowski, R. E.
College/School:College of Science and Engineering > School of Physics and Astronomy
Research Group:Materials and Condensed Matter Physics
Journal Name:Journal of the Royal Society: Interface
Publisher:The Royal Society
ISSN:1742-5689
ISSN (Online):1742-5689
Published Online:11 October 2017
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Journal of the Royal Society: Interface 14(135): 20170464
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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