Baran-Gale, J., Chandra, T. and Kirschner, K. (2018) Experimental design for single-cell RNA sequencing. Briefings in Functional Genomics, 17(4), pp. 233-239. (doi: 10.1093/bfgp/elx035) (PMID:29126257) (PMCID:PMC6063265)
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Abstract
Single-cell RNA sequencing (scRNA-seq) has opened new avenues for the characterization of heterogeneity in a large variety of cellular systems. As this is a relatively new technique, the field is fast evolving. Here, we discuss general considerations in experimental design and the two most popular approaches, plate-based Smart-Seq2 and microdroplet-based scRNA-seq at the example of 10x Chromium. We discuss advantages and disadvantages of both methods and point out major factors to consider in designing successful experiments.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Kirschner, Dr Kristina |
| Authors: | Baran-Gale, J., Chandra, T., and Kirschner, K. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
| Journal Name: | Briefings in Functional Genomics |
| Publisher: | Oxford University Press |
| ISSN: | 2041-2649 |
| ISSN (Online): | 2041-2657 |
| Published Online: | 08 November 2017 |
| Copyright Holders: | Copyright © 2017 The Authors |
| First Published: | First published in Briefings in Functional Genomics 17(4): 233-239 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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