Experimental design for single-cell RNA sequencing

Baran-Gale, J., Chandra, T. and Kirschner, K. (2018) Experimental design for single-cell RNA sequencing. Briefings in Functional Genomics, 17(4), pp. 233-239. (doi: 10.1093/bfgp/elx035) (PMID:29126257) (PMCID:PMC6063265)

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Single-cell RNA sequencing (scRNA-seq) has opened new avenues for the characterization of heterogeneity in a large variety of cellular systems. As this is a relatively new technique, the field is fast evolving. Here, we discuss general considerations in experimental design and the two most popular approaches, plate-based Smart-Seq2 and microdroplet-based scRNA-seq at the example of 10x Chromium. We discuss advantages and disadvantages of both methods and point out major factors to consider in designing successful experiments.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Kirschner, Dr Kristina
Authors: Baran-Gale, J., Chandra, T., and Kirschner, K.
College/School:College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Journal Name:Briefings in Functional Genomics
Publisher:Oxford University Press
ISSN (Online):2041-2657
Published Online:08 November 2017
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Briefings in Functional Genomics 17(4): 233-239
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
632234Funding SchemesAnna DominiczakWellcome Trust (WELLCOTR)105614/Z/14/ZRI CARDIOVASCULAR & MEDICAL SCIENCES