The microevolution and epidemiology of Staphylococcus aureus colonization during atopic eczema disease flare

Harkins, C. P. et al. (2018) The microevolution and epidemiology of Staphylococcus aureus colonization during atopic eczema disease flare. Journal of Investigative Dermatology, 138(2), pp. 336-343. (doi: 10.1016/j.jid.2017.09.023) (PMID:28951239) (PMCID:PMC5780352)

[img]
Preview
Text
149568.pdf - Published Version
Available under License Creative Commons Attribution.

415kB

Abstract

Staphylococcus aureus is an opportunistic pathogen and variable component of the human microbiota. In atopic eczema (AE) a characteristic of the disease is colonization by S. aureus, with exacerbations associated with an increased bacterial burden of the organism. Despite this, the origins and genetic diversity of S. aureus colonizing individual patients during AE disease flares is poorly understood. To examine the micro-evolution of S. aureus colonization we have deep-sequenced S. aureus populations from nine children with moderate to severe AE, and 18 non-atopic children asymptomatically carrying S. aureus nasally. Colonization by clonal S. aureus populations was observed in both AE cases and controls, with all but one of the individuals containing colonies belonging to a single sequence type. Phylogenetic analysis revealed that disease flares were associated with the clonal expansion of the S. aureus population, occurring over a period of weeks to months. There was a significant difference in the genetic backgrounds of S. aureus colonizing AE patients versus controls (Fisher's Exact test, p=0.03). Examination of intra-host genetic heterogeneity of the colonizing S. aureus populations identified evidence of within-host selection in the AE patients, with AE variants being potentially selectively advantageous for intracellular persistence and treatment resistance.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Oravcova, Dr Katarina
Authors: Harkins, C. P., Pettigrew, K. A., Oravcová, K., Gardner, J., Hearn, R.M. R., Rice, D., Mather, A. E., Parkhill, J., Brown, S. J., Proby, C. M., and Holden, M. T.G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Journal of Investigative Dermatology
Publisher:Elsevier
ISSN:0022-202X
ISSN (Online):1523-1747
Published Online:23 September 2017
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Journal of Investigative Dermatology 138(2): 336-343
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record