Optically Enhanced Acoustophoresis

McDougall, C., O'Mahoney, P., McGuinn, A., Willoughby, N. A., Qiu, Y. , Demore, C. E. M. and Spalding, G. C. (2017) Optically Enhanced Acoustophoresis. In: SPIE Nanoscience + Engineering, San Diego, CA, USA, 6-10 Aug 2017, 103471B-1. (doi: 10.1117/12.2276323)

Abstract

Regenerative medicine has the capability to revolutionise many aspects of medical care, but for it to make the step from small scale autologous treatments to larger scale allogeneic approaches, robust and scalable label free cell sorting technologies are needed as part of a cell therapy bioprocessing pipeline. In this proceedings we describe several strategies for addressing the requirements for high throughput without labeling via: dimensional scaling, rare species targeting and sorting from a stable state. These three approaches are demonstrated through a combination of optical and ultrasonic forces. By combining mostly conservative and non-conservative forces from two different modalities it is possible to reduce the influence of flow velocity on sorting efficiency, hence increasing robustness and scalability. One such approach can be termed "optically enhanced acoustophoresis" which combines the ability of acoustics to handle large volumes of analyte with the high specificity of optical sorting.

• Item Type: Conference Proceedings
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Qiu, Dr Yongqiang
• Authors: McDougall, C., O'Mahoney, P., McGuinn, A., Willoughby, N. A., Qiu, Y., Demore, C. E. M., and Spalding, G. C.
• College/School: College of Science and Engineering > School of Engineering > Systems Power and Energy
• ISSN: 0277-786X
• Copyright Holders: Copyright © 2017 The AuthorsSociety of Photo Optical Instrumentation Engineers (SPIE)
• First Published: First published in Proceedings of the SPIE 10347: 103471B-1
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher.