Enhanced surface acoustic wave cell sorting by 3D microfluidic chip design

Ung, W.L., Mutafopulos, K., Spink, P., Rambach, R.W., Franke, T. and Weitz, D.A. (2017) Enhanced surface acoustic wave cell sorting by 3D microfluidic chip design. Lab on a Chip, 17(23), pp. 4059-4069. (doi:10.1039/C7LC00715A)

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Abstract

We demonstrate an acoustic wave driven microfluidic cell sorter that combines advantages of multilayer device fabrication with planar surface acoustic wave excitation. We harness the strong vertical component of the refracted acoustic wave to enhance cell actuation by using an asymmetric flow field to increase cell deflection. Precise control of the 3-dimensional flow is realized by topographical structures implemented on the top of the microchannel. We experimentally quantify the effect of the structure dimensions and acoustic parameter. The design attains cell sorting rates and purities approaching those of state of the art fluorescence-activated cell sorters with all the advantages of microfluidic cell sorting.

Item Type:Articles
Additional Information:This work was supported by the National Science Foundation (DMR-1310266), the Harvard Materials Research Science and Engineering Center (DMR-1420570), and the Defense Advanced Research Projects Agency (HR0011-11-C-0093). This work was performed in part at the Center for Nanoscale Systems (CNS), a member of the National Nanotechnology Infrastructure Network (NNIN), which is supported by the National Science Foundation under NSF award no. ECS- 0335765.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Franke, Professor Thomas
Authors: Ung, W.L., Mutafopulos, K., Spink, P., Rambach, R.W., Franke, T., and Weitz, D.A.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Lab on a Chip
Publisher:Royal Society of Chemistry
ISSN:1473-0197
ISSN (Online):1473-0189
Published Online:03 October 2017
Copyright Holders:Copyright © 2017 Royal Society of Chemistry
First Published:First published in Lab on a Chip 17(23):4059-4069
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher.

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