Spatial intensity distribution analysis: studies of G Protein-coupled receptor oligomerization

Pediani, J. D. , Ward, R. J., Marsango, S. and Milligan, G. (2018) Spatial intensity distribution analysis: studies of G Protein-coupled receptor oligomerization. Trends in Pharmacological Sciences, 39(2), pp. 175-186. (doi: 10.1016/j.tips.2017.09.001) (PMID:29032835) (PMCID:PMC5783713)

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Abstract

Spatial intensity distribution analysis (SpIDA) is a recently developed approach for determining quaternary structure information on fluorophore-labelled proteins of interest in situ. It can be applied to live or fixed cells and native tissue. Using confocal images, SpIDA generates fluorescence intensity histograms that are analysed by super-Poissonian distribution functions to obtain density and quantal brightness values of the fluorophore-labelled protein of interest. This allows both expression level and oligomerisation state of the protein to be determined. We describe the application of SpIDA to investigate the oligomeric state of G protein-coupled receptors (GPCRs) at steady state and following cellular challenge, and consider how SpIDA may be used to explore GPCR quaternary organisation in pathophysiology and to stratify medicines.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Pediani, Dr John and Ward, Dr Richard and Milligan, Professor Graeme and Marsango, Dr Sara
Authors: Pediani, J. D., Ward, R. J., Marsango, S., and Milligan, G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Trends in Pharmacological Sciences
Publisher:Elsevier
ISSN:0165-6147
ISSN (Online):1873-3735
Published Online:06 October 2017
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Trends in Pharmacological Sciences 39(2):175-186
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
656901The organisational structure of class A GPCRs: Implications for pharmacology, function and therapeutic regulationGraeme MilliganMedical Research Council (MRC)MR/L023806/1RI MOLECULAR CELL & SYSTEMS BIOLOGY