The functional response of mesenchymal stem cells to electron-beam patterned elastomeric surfaces presenting micrometer to nanoscale heterogeneous rigidity

Biggs, M. J.P. et al. (2017) The functional response of mesenchymal stem cells to electron-beam patterned elastomeric surfaces presenting micrometer to nanoscale heterogeneous rigidity. Advanced Materials, 29(39), 1702119. (doi:10.1002/adma.201702119) (PMID:28861921)

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Abstract

Cells directly probe and respond to the physicomechanical properties of their extracellular environment, a dynamic process which has been shown to play a key role in regulating both cellular adhesive processes and differential cellular function. Recent studies indicate that stem cells show lineage-specific differentiation when cultured on substrates approximating the stiffness profiles of specific tissues. Although tissues are associated with a range of Young's modulus values for bulk rigidity, at the subcellular level, tissues are comprised of heterogeneous distributions of rigidity. Lithographic processes have been widely explored in cell biology for the generation of analytical substrates to probe cellular physicomechanical responses. In this work, it is shown for the first time that that direct-write e-beam exposure can significantly alter the rigidity of elastomeric poly(dimethylsiloxane) substrates and a new class of 2D elastomeric substrates with controlled patterned rigidity ranging from the micrometer to the nanoscale is described. The mechanoresponse of human mesenchymal stem cells to e-beam patterned substrates was subsequently probed in vitro and significant modulation of focal adhesion formation and osteochondral lineage commitment was observed as a function of both feature diameter and rigidity, establishing the groundwork for a new generation of biomimetic material interfaces.

Item Type:Articles
Keywords:Mechanical engineering, general materials science, mechanics of materials.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Wheadon, Dr Helen
Authors: Biggs, M. J.P., Fernandez, M., Thomas, D., Cooper, R., Palma, M., Liao, J., Fazio, T., Dahlberg, C., Wheadon, H., Pallipurath, A., Pandit, A., Kysar, J., and Wind, S. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Advanced Materials
Publisher:Wiley
ISSN:0935-9648
ISSN (Online):1521-4095
Published Online:01 September 2017
Copyright Holders:Copyright © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
First Published:First published in Advanced Materials 29(39): 1702119
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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