Conformational heterogeneity in a fully-complementary DNA three-way junction with a GC-rich branchpoint

Magennis, S., Toulmin, A., Baltierra-Jasso, L. E., Morten, M. J., Sabir, T., McGlynn, P., Schröder, G. F., Smith, B. O. and Magennis, S. W. (2017) Conformational heterogeneity in a fully-complementary DNA three-way junction with a GC-rich branchpoint. Biochemistry, 56(37), pp. 4985-4991. (doi:10.1021/acs.biochem.7b00677) (PMID:28820590)

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Abstract

DNA three-way junctions (3WJs) are branched structures that serve as important biological intermediates and as components in DNA nanostructures. We recently derived the global structure of a fully-complementary 3WJ and found that it contained unpaired bases at the branchpoint, in support of previous observations of branch flexibility and branchpoint reactivity. By combining high-resolution single-molecule FRET, molecular modeling, time-resolved ensemble fluorescence spectroscopy and the first 19F NMR observations of fully complementary 3WJs, we now show that the 3WJ structure can adopt multiple distinct conformations depending upon the sequence at the branchpoint. A 3WJ with a GC-rich branchpoint adopts an open conformation with unpaired bases at the branch and at least one additional conformation with increased base interactions at the branchpoint. This structural diversity has implications for branch interactions and processing in vivo and for technological applications.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Magennis, Dr Steven and Smith, Dr Brian and Morten, Dr Michael
Authors: Magennis, S., Toulmin, A., Baltierra-Jasso, L. E., Morten, M. J., Sabir, T., McGlynn, P., Schröder, G. F., Smith, B. O., and Magennis, S. W.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Chemistry
Journal Name:Biochemistry
Publisher:American Chemical Society
ISSN:0006-2960
ISSN (Online):1520-4995
Published Online:18 August 2017
Copyright Holders:Copyright © 2017 American Chemical Society
First Published:First published in Biochemistry 56(37): 4985-4991
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
657181From nature to nano: structure, dynamics and reactivity of DNA three-way junctionsSteven MagennisEngineering and Physical Sciences Research Council (EPSRC)EP/L027003/1SCHOOL OF CHEMISTRY