The potential for reassortment between Oropouche and Schmallenberg Orthobunyaviruses

Tilston-Lunel, N. L., Shi, X. , Elliott, R. M. and Olszanski Acrani, G. (2017) The potential for reassortment between Oropouche and Schmallenberg Orthobunyaviruses. Viruses, 9(8), 220. (doi: 10.3390/v9080220) (PMID:28800086) (PMCID:PMC5580477)

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Abstract

A number of viruses within the Peribunyaviridae family are naturally occurring reassortants, a common phenomenon for segmented viruses. Using a minigenome-reporter and virus-like particle (VLP) production assay, we have accessed the potential of Oropouche virus (OROV), Schmallenberg virus (SBV), and other orthobunyaviruses within the Simbu serogroup to reassort. We found that the untranslated region (UTR) in the medium segment is a potential contributing factor for reassortment by the tested viruses. We demonstrate that for promoter activity to occur it was essential that the viral RNA polymerase (L) and nucleocapsid (N) proteins were from the same virus, reinforcing the hypothesis that the large and small segments that encode these proteins segregate together during genome reassortment. Our results indicate that, given the right epidemiological setting, reassortment between SBV and OROV would potentially be feasible and could contribute to the emergence of a new Simbu virus.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Olszanski Acrani, Mr Gustavo and Elliott, Professor Richard and Shi, Dr Xiaohong
Authors: Tilston-Lunel, N. L., Shi, X., Elliott, R. M., and Olszanski Acrani, G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Viruses
Publisher:MDPI
ISSN:1999-4915
ISSN (Online):1999-4915
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Viruses 9(8):220
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
635361Molecular analyses of arbovirus-host interactionsMassimo PalmariniWellcome Trust (WELLCOTR)099220/B/12/ZMVLS III - CENTRE FOR VIRUS RESEARCH