Stimulation of 3D osteogenesis by mesenchymal stem cells using a nanovibrational bioreactor

Tsimbouri, P. M. et al. (2017) Stimulation of 3D osteogenesis by mesenchymal stem cells using a nanovibrational bioreactor. Nature Biomedical Engineering, 1, pp. 758-770. (doi:10.1038/s41551-017-0127-4)

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Abstract

Bone grafts are one of the most commonly transplanted tissues. However, autologous grafts are in short supply, and can be associated with pain and donor-site morbidity. The creation of tissue-engineered bone grafts could help to fulfil clinical demand and provide a crucial resource for drug screening. Here, we show that vibrations of nanoscale amplitude provided by a newly developed bioreactor can differentiate a potential autologous cell source, mesenchymal stem cells (MSCs), into mineralized tissue in 3D. We demonstrate that nanoscale mechanotransduction can stimulate osteogenesis independently of other environmental factors, such as matrix rigidity. We show this by generating mineralized matrix from MSCs seeded in collagen gels with stiffness an order of magnitude below the stiffness of gels needed to induce bone formation in vitro. Our approach is scalable and can be compatible with 3D scaffolds.

Item Type:Articles
Additional Information:Also funded by EP/N012631/1 to UWS
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Curtis, Professor Adam and Tsimbouri, Dr Penelope and Salmeron-Sanchez, Professor Manuel and Dalby, Professor Matthew and Gonzalez Garcia, Dr Cristina and Blackburn, Dr Gavin and Burgess, Dr Karl and Jayawarna, Dr Vineetha and Childs, Dr Peter and Yang, Dr Jingli
Authors: Tsimbouri, P. M., Childs, P. G., Pemberton, G. D., Yang, J., Jayawarna, V., Oripiriyakul, W., Burgess, K., Gonzalez-Garcia, C., Blackburn, G., Thomas, D., Giraldo, C. V., Biggs, M. J.P., Curtis, A. S.G., Salmeron-Sanchez, M., Reid, S., and Dalby, M. J.
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Nature Biomedical Engineering
Publisher:Nature Publishing Group
ISSN:2157-846X
ISSN (Online):2157-846X
Copyright Holders:Copyright © 2017 Macmillan Publishers Limited, part of Springer Nature
First Published:First published in Nature Biomedical Engineering 1:758-770
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
709981Development of NanoKick BioreactorMatthew DalbyBiotechnology and Biological Sciences Research Council (BBSRC)BB/N012690/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
728381Rapid Bone Graft Synthesis Through Dual Piezoelectric/Nanomechaniocal StimulationMatthew DalbyBiotechnology and Biological Sciences Research Council (BBSRC)BB/P00220X/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
691891Developing the NanoKick bioreactor to enable tissue engineered bone graft and use of metabolomics to identify bone specific drug candidatesMatthew DalbyEngineering and Physical Sciences Research Council (EPSRC)EP/N013905/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
722061Engineering growth factor microenvironments- a new therapeutic paradigm for regenerative medicineManuel Salmeron-SanchezEngineering and Physical Sciences Research Council (EPSRC)EP/P001114/1ENG - BIOMEDICAL ENGINEERING