MicroRNA29a treatment improves early tendon injury

Watts, A. E. et al. (2017) MicroRNA29a treatment improves early tendon injury. Molecular Therapy, 25(10), pp. 2415-2426. (doi:10.1016/j.ymthe.2017.07.015) (PMID:28822690)

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Abstract

Tendon injuries (tendinopathies) are common in human and equine athletes and characterised by dysregulated collagen matrix resulting in tendon damage. We have previously demonstrated a functional role for miR29a as a post-transcriptional regulator of collagen 3 expression in murine and human tendon injury. Given the translational potential, we designed a randomised, blinded trial to evaluate the potential of a miR29a replacement therapy as a therapeutic option to treat tendinopathy in an equine model which closely mimics human disease. Tendon injury was induced in the superficial digital flexor tendon (SDFT) of 17 horses. Tendon lesions were treated one week later with an intralesional injection of miR29a or placebo. miR29a treatment reduced collagen 3 transcript levels at week 2 with no significant changes in collagen 1. Relative lesion cross-sectional area was significantly lower in miR29a tendons compared to control tendons. Histology scores were significantly better for miR29a treated tendons compared to control tendons. This data supports the mechanism of miRNA-mediated modulation of the early pathophysiologic events that facilitate tissue remodelling in the tendon after injury and provides strong proof of principle that a locally delivered miR29a therapy improves early tendon healing.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McInnes, Professor Iain and Akbar, Mr Moeed and Kitson, Miss Susan and Gilchrist, Dr Derek and Millar, Dr Neal
Authors: Watts, A. E., Millar, N. L., Platt, J., Kitson, S. M., Akbar, M., Rech, R., Griffin, J., Pool, R., Hughes, T., McInnes, I. B., and Gilchrist, D.S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Molecular Therapy
Publisher:Elsevier
ISSN:1525-0016
ISSN (Online):1525-0024
Published Online:28 July 2017
Copyright Holders:Copyright © 2017 The American Society of Gene and Cell Therapy
First Published:First published in Molecular Therapy 25(10): 2415-2426
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
690421Glasgow Molecular Pathology (GMP) NodeKarin OienMedical Research Council (MRC)MR/N005813/1ICS - EXPERIMENTAL THERAPEUTICS