Identification of an essential virulence gene of cyprinid herpesvirus 3

Boutier, M., Gao, Y., Vancsok, C., Suárez, N., Davison, A. J. and Vanderplasschen, A. (2017) Identification of an essential virulence gene of cyprinid herpesvirus 3. Antiviral Research, 145, pp. 60-69. (doi:10.1016/j.antiviral.2017.07.002) (PMID:28690142) (PMCID:PMC5588920)

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Abstract

The genus Cyprinivirus consists of a growing list of phylogenetically related viruses, some of which cause severe economic losses to the aquaculture industry. The archetypal member, cyprinid herpesvirus 3 (CyHV-3) causes mass mortalities worldwide in koi and common carp. A CyHV-3 mutant was described previously that is attenuated in vivo by a deletion affecting two genes (ORF56 and ORF57). The relative contributions of ORF56 and ORF57 to the safety and efficacy profile of this vaccine candidate have now been assessed by analysing viruses individually deleted for ORF56 or ORF57. Inoculation of these viruses into carp demonstrated that the absence of ORF56 did not affect virulence, whereas the absence of ORF57 led to an attenuation comparable to, though slightly less than, that of the doubly deleted virus. To demonstrate further the role of ORF57 as a key virulence factor, a mutant retaining the ORF57 region but unable to express the ORF57 protein was produced by inserting multiple in-frame stop codons into the coding region. Analysis of this virus in vivo revealed a safety and efficacy profile comparable to that of the doubly deleted virus. These findings show that ORF57 encodes an essential CyHV-3 virulence factor. They also indicate that ORF57 orthologues in other cypriniviruses may offer promising targets for the rational design of attenuated recombinant vaccines.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Suarez, Dr Nicolas and Davison, Professor Andrew
Authors: Boutier, M., Gao, Y., Vancsok, C., Suárez, N., Davison, A. J., and Vanderplasschen, A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Antiviral Research
Publisher:Elsevier
ISSN:0166-3542
ISSN (Online):1872-9096
Published Online:06 July 2017
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Antiviral Research 145: 60-69
Publisher Policy:Reproduced under a Creative Commons license

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
656321Genomics of human cytomegalovirusAndrew DavisonMedical Research Council (MRC)MC_UU_12014/3MVLS III - CENTRE FOR VIRUS RESEARCH