Visual detection of Brucella in bovine biological samples using DNA-activated gold nanoparticles

Pal, D., Boby, N., Kumar, S., Kaur, G., Ali, S. A., Reboud, J. , Shrivastava, S., Gupta, P. K., Cooper, J. M. and Chaudhuri, P. (2017) Visual detection of Brucella in bovine biological samples using DNA-activated gold nanoparticles. PLoS ONE, 12(7), e0180919. (doi:10.1371/journal.pone.0180919) (PMID:28719613) (PMCID:PMC5515423)

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Abstract

Brucellosis is a bacterial disease, which, although affecting cattle primarily, has been associated with human infections, making its detection an important challenge. The existing gold standard diagnosis relies on the culture of bacteria which is a lengthy and costly process, taking up to 45 days. New technologies based on molecular diagnosis have been proposed, either through dip-stick, immunological assays, which have limited specificity, or using nucleic acid tests, which enable to identify the pathogen, but are impractical for use in the field, where most of the reservoir cases are located. Here we demonstrate a new test based on hybridization assays with metal nanoparticles, which, upon detection of a specific pathogen-derived DNA sequence, yield a visual colour change. We characterise the components used in the assay with a range of analytical techniques and show sensitivities down to 1000 cfu/ml for the detection of Brucella. Finally, we demonstrate that the assay works in a range of bovine samples including semen, milk and urine, opening up the potential for its use in the field, in low-resource settings.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cooper, Professor Jonathan and Reboud, Dr Julien
Authors: Pal, D., Boby, N., Kumar, S., Kaur, G., Ali, S. A., Reboud, J., Shrivastava, S., Gupta, P. K., Cooper, J. M., and Chaudhuri, P.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:PLoS ONE
Publisher:Public Library of Science
ISSN:1932-6203
ISSN (Online):1932-6203
Copyright Holders:Copyright © 2017 Pal et al.
First Published:First published in PLoS ONE 12(7): e0180919
Publisher Policy:Reproduced under a Creative Commons license

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
634061Development of multiplexed diagnostic biosensor for infectious reproductive diseases of cattle and buffaloesJonathan CooperBiotechnology and Biological Sciences Research Council (BBSRC)BB/L00464X/1ENG - BIOMEDICAL ENGINEERING