Santos, R. E.R.S., Silva, G. L.A., Santos, E. V., Duncan, S. M., Mottram, J. C. , Damasceno, J. D. and Tosi, L. R.O. (2017) A DiCre recombinase-based system for inducible expression in Leishmania major. Molecular and Biochemical Parasitology, 216, pp. 45-48. (doi: 10.1016/j.molbiopara.2017.06.006) (PMID:28629935)
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Abstract
Here we present the establishment of an inducible system based on the dimerizable Cre recombinase (DiCre) for controlled gene expression in the protozoan parasite Leishmania. Rapamycin-induced DiCre activation promoted efficient flipping and expression of gene products in a time and dose-dependent manner. The DiCre flipping activity induced the expression of target genes from both integrated and episomal contexts broadening the applicability of the system. We validated the system by inducing the expression of both full length and truncated forms of the checkpoint protein Rad9, which revealed that the highly divergent C-terminal domain of Rad9 is necessary for proper subcellular localization. Thus, by establishing the DiCre-based inducible system we have created and validated a robust new tool for assessing gene function in Leishmania.
| Item Type: | Articles |
|---|---|
| Keywords: | 9-1-1 complex, DNA damage response, DiCre recombinase, Leishmania, Rad9-Rad1-Hus1, inducible expression. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Duncan, Mr Samuel and Damasceno, Dr Jeziel and Mottram, Professor Jeremy |
| Authors: | Santos, R. E.R.S., Silva, G. L.A., Santos, E. V., Duncan, S. M., Mottram, J. C., Damasceno, J. D., and Tosi, L. R.O. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Molecular and Biochemical Parasitology |
| Publisher: | Elsevier |
| ISSN: | 0166-6851 |
| ISSN (Online): | 1872-9428 |
| Published Online: | 16 June 2017 |
| Copyright Holders: | Copyright © 2017 Elsevier B.V. |
| First Published: | First published in Molecular and Biochemical Parasitology 216: 45-48 |
| Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
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