Improving cartilage phenotype from differentiated pericytes in tunable peptide hydrogels

Alakpa, E. V., Jayawarna, V., Burgess, K. E.V., West, C. C., Péault, B., Ulijn, R. V. and Dalby, M. J. (2017) Improving cartilage phenotype from differentiated pericytes in tunable peptide hydrogels. Scientific Reports, 7, 6895. (doi:10.1038/s41598-017-07255-z) (PMID:28761049) (PMCID:PMC5537289)

[img]
Preview
Text
143128.pdf - Published Version
Available under License Creative Commons Attribution.

2MB

Abstract

Differentiation of stem cells to chondrocytes in vitro usually results in a heterogeneous phenotype. This is evident in the often detected over expression of type X collagen which, in hyaline cartilage structure is not characteristic of the mid-zone but of the deep-zone ossifying tissue. Methods to better match cartilage developed in vitro to characteristic in vivo features are therefore highly desirable in regenerative medicine. This study compares phenotype characteristics between pericytes, obtained from human adipose tissue, differentiated using diphenylalanine/serine (F2/S) peptide hydrogels with the more widely used chemical induced method for chondrogenesis. Significantly higher levels of type II collagen were noted when pericytes undergo chondrogenesis in the hydrogel in the absence of induction media. There is also a balanced expression of collagen relative to aggrecan production, a feature which was biased toward collagen production when cells were cultured with induction media. Lastly, metabolic profiles of each system show considerable overlap between both differentiation methods but subtle differences which potentially give rise to their resultant phenotype can be ascertained. The study highlights how material and chemical alterations in the cellular microenvironment have wide ranging effects on resultant tissue type.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Burgess, Dr Karl and Jayawarna, Dr Vineetha and Dalby, Professor Matthew
Authors: Alakpa, E. V., Jayawarna, V., Burgess, K. E.V., West, C. C., Péault, B., Ulijn, R. V., and Dalby, M. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Scientific Reports
Publisher:Nature Publishing Group
ISSN:2045-2322
ISSN (Online):2045-2322
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Scientific Reports 7: 6895
Publisher Policy:Reproduced under a Creative Commons license

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
470561DTC in cell and proteomic technologies (continuation)Jonathan CooperEngineering and Physical Sciences Research Council (EPSRC)EP/F500424/1ENG - BIOMEDICAL ENGINEERING
608751Dynamic surfaces to mimic mesenchymal stem cell niche functionsMatthew DalbyBiotechnology and Biological Sciences Research Council (BBSRC)BB/K006908/1RI MOLECULAR CELL & SYSTEMS BIOLOGY