Duncan, S. M., Jones, N. G. and Mottram, J. C. (2017) Recent advances in Leishmania reverse genetics: manipulating a manipulative parasite. Molecular and Biochemical Parasitology, 216, pp. 30-38. (doi: 10.1016/j.molbiopara.2017.06.005) (PMID:28629934)
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Abstract
In this review we describe the expanding repertoire of molecular tools with which to study gene function in Leishmania. Specifically we review the tools available for studying functions of essential genes, such as plasmid shuffle and DiCre, as well as the rapidly expanding portfolio of available CRISPR/Cas9 approaches for large scale gene knockout and endogenous tagging. We include detail on approaches that allow the direct manipulation of RNA using RNAi and protein levels via Tet or DiCre induced overexpression and destabilization domain mediated degradation. The utilisation of current methods and the development of more advanced molecular tools will lead to greater understanding of the role of essential genes in the parasite and thereby more robust drug target validation, thereby paving the way for the development of novel therapeutics to treat this important disease.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Duncan, Mr Samuel and Jones, Mr Nathaniel and Mottram, Professor Jeremy |
| Authors: | Duncan, S. M., Jones, N. G., and Mottram, J. C. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Molecular and Biochemical Parasitology |
| Publisher: | Elsevier |
| ISSN: | 0166-6851 |
| ISSN (Online): | 1872-9428 |
| Published Online: | 16 June 2017 |
| Copyright Holders: | Copyright © 2017 The Authors |
| First Published: | First published in Molecular and Biochemical Parasitology 216: 30-38 |
| Publisher Policy: | Reproduced under a Creative Commons license |
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