Kinetics of internalisation and degradation of surface-bound interferon in human lymphoblastoid cells

Evans, T. and secher, D. (1984) Kinetics of internalisation and degradation of surface-bound interferon in human lymphoblastoid cells. EMBO Journal, 3(12), pp. 2975-2978. (PMID:6241148) (PMCID:PMC557800)

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Abstract

The binding of iodinated human interferon-alpha 2 (IFN-alpha 2) was studied on the human T cell line, Molt 4. After its initial binding to cells, the IFN is transferred to a trypsin-resistant compartment before appearing in the medium as TCA-soluble material, while the total cell-associated IFN declines to one-third of its maximum value after 3 h incubation. The Na+/H+ ionophore monensin did not prevent intracellular accumulation of IFN but did completely inhibit its breakdown. We interpret our results as evidence for receptor-mediated internalisation of IFN followed by intracellular breakdown.

Item Type:Articles
Refereed:No
Glasgow Author(s) Enlighten ID:Evans, Professor Tom
Authors: Evans, T., and secher, D.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:EMBO Journal
Publisher:EMBO Press
ISSN:0261-4189
ISSN (Online):1460-2075

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