The mechanism of action of interferon-α (IFN-α) in hairy-cell leukaemia; Hu-IFN-α2 receptor expression by hairy cells and other normal and leukaemic cell types

Dadmarz, R., Evans, T., Secher, D., Marshall, N. and Cawley, J. (1986) The mechanism of action of interferon-α (IFN-α) in hairy-cell leukaemia; Hu-IFN-α2 receptor expression by hairy cells and other normal and leukaemic cell types. Leukemia Research, 10(11), pp. 1279-1285.

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Abstract

To investigate the possible direct effects of interferon-α (IFN-α) in hairy-cell leukaemia, IFN-α receptor expression by hairy cells (HCs) (11 cases) was measured by a radiolabelling technique and compared with that of MOLT-4, chronic lymphocytic leukaemia (CLL; 14 cases) and various other leukaemic and normal cell types. Purified peripheral blood (PB) and splenic HCs showed higher levels of receptor expression (approx. 1000 ± 200 binding sites/cell; 11 cases tested) than other normal and leukaemic cells types. Purified normal PB and tonsil B cells showed low levels of receptors (approx. 120 ± 100 binding sites/cell), while a range of B-cell leukaemias displayed intermediate levels of expression (approx. 100–500 sites/cell). In the 15 cases of CLL tested, 530 ± 330 binding sites/cell were demonstrated, the high standard deviation reflecting the fact that approximately one third of cases had receptor levels comparable with those in HCL. Normal and HCL T cells, red cells and platelets had no demonstrable IFN receptors. It is suggested that these findings may be relevant to the efficacy of IFN in hairy-cell leukaemia.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Evans, Professor Tom
Authors: Dadmarz, R., Evans, T., Secher, D., Marshall, N., and Cawley, J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Leukemia Research
Publisher:Elsevier
ISSN:0145-2126
ISSN (Online):1873-5835
Published Online:01 March 2004

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