Confined sandwichlike microenvironments tune myogenic differentiation

Ballester-Beltrán, J., Trujillo, S. , Alakpa, E. V., Compañ, V., Gavara, R., Meek, D., West, C. C., Péault, B., Dalby, M. J. and Salmeron-Sanchez, M. (2017) Confined sandwichlike microenvironments tune myogenic differentiation. ACS Biomaterials Science and Engineering, 3(8), pp. 1710-1718. (doi:10.1021/acsbiomaterials.7b00109) (PMID:28824958) (PMCID:PMC5558191)

[img]
Preview
Text
142310.pdf - Published Version
Available under License Creative Commons Attribution.

8MB

Abstract

Sandwichlike (SW) cultures are engineered as a multilayer technology to simultaneously stimulate dorsal and ventral cell receptors, seeking to mimic cell adhesion in three-dimensional (3D) environments in a reductionist manner. The effect of this environment on cell differentiation was investigated for several cell types cultured in standard growth media, which promotes proliferation on two-dimensional (2D) surfaces and avoids any preferential differentiation. First, murine C2C12 myoblasts showed specific myogenic differentiation. Human mesenchymal stem cells (hMSCs) of adipose and bone marrow origin, which can differentiate toward a wider variety of lineages, showed again myodifferentiation. Overall, this study shows myogenic differentiation in normal growth media for several cell types under SW conditions, avoiding the use of growth factors and cytokines, i.e., solely by culturing cells within the SW environment. Mechanistically, it provides further insights into the balance between integrin adhesion to the dorsal substrate and the confinement imposed by the SW system.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel and Dalby, Professor Matthew and Alakpa, Miss Enateri and Meek, Professor Dominic and Trujillo-Muñoz, Sara
Authors: Ballester-Beltrán, J., Trujillo, S., Alakpa, E. V., Compañ, V., Gavara, R., Meek, D., West, C. C., Péault, B., Dalby, M. J., and Salmeron-Sanchez, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering
College of Science and Engineering > School of Engineering > Biomedical Engineering
College of Science and Engineering > School of Geographical and Earth Sciences
Journal Name:ACS Biomaterials Science and Engineering
Publisher:American Chemical Society
ISSN:2373-9878
ISSN (Online):2373-9878
Published Online:09 June 2017
Copyright Holders:Copyright © 2017 American Chemical Society
First Published:First published in ACS Biomaterials Science and Engineering 3(8): 1710-1718
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
722061Engineering growth factor microenvironments- a new therapeutic paradigm for regenerative medicineManuel Salmeron-SanchezEngineering and Physical Sciences Research Council (EPSRC)EP/P001114/1ENG - BIOMEDICAL ENGINEERING
626901HEALINSYNERGY - Material-driven fibronectin fibrillogenesis to engineer synergistic growth factor microenvironmentsManuel Salmeron-SanchezEuropean Research Council (ERC)306990ENG - BIOMEDICAL ENGINEERING