The role of lipopolysaccharide, pro-inflammatory cytokines and bacterial superantigens in the transcriptional regulation of lymphotoxin α and β in mouse splenocytes

Zinetti, M., Agyekum, S., Evans, T. , Polak, J. and Cohen, J. (1998) The role of lipopolysaccharide, pro-inflammatory cytokines and bacterial superantigens in the transcriptional regulation of lymphotoxin α and β in mouse splenocytes. Cytokine, 10(12), pp. 940-947. (doi: 10.1006/cyto.1998.0386)

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Abstract

Lymphotoxin α (LT-α) and lymphotoxin β (LT-β) are members of the tumour necrosis factor (TNF) ligand family. Because of the importance of TNF in the pathogenesis of septic shock, the expression of LT-α and LT-β mRNA in murine splenocytes stimulated with different pro-inflammatory cytokines, sepsis-associated mediators such as lipopolysaccharide (LPS) and bacterial superantigens was investigated. The authors show that the bacterial superantigens, toxic shock syndrome toxin 1 (TSST-1) and staphylococcal enterotoxin B (SEB) upregulate LT-α mRNA expression in vitro in murine cells. Basal expression of LT-β mRNA was found in unstimulated murine splenocytes, and could be increased by the addition of the mitogen concanavalin A (Con A). Despite this suggested inducibility of the murine LT-β transcript, sepsis-associated mediators did not affect its regulation. Neither the pro-inflammatory cytokines interleukin 2 (IL-2), TNF-α nor LPS alone or in combination with interferon γ (IFN-γ) had any effect on LT-β mRNA expression. The bacterial superantigens TSST-1, SEB and streptococcal pyrogenic exotoxin A (SPEA) were also unable to upregulate LT-β mRNA transcript, in contrast to the observation with LT-α.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Evans, Professor Tom
Authors: Zinetti, M., Agyekum, S., Evans, T., Polak, J., and Cohen, J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Cytokine
Publisher:Elsevier
ISSN:1043-4666
ISSN (Online):1096-0023

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