Dielectrophoretic manipulation and characterization of herpes simplex virus-1 capsids

Hughes, M.P., Morgan, H. and Rixon, F.J. (2001) Dielectrophoretic manipulation and characterization of herpes simplex virus-1 capsids. European Biophysics Journal with Biophysics Letters, 30, pp. 268-272. (doi: 10.1007/s002490100144)

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Abstract

The dielectrophoretic behaviour of the capsids of herpes simplex virus type-1 has been measured over a range of conductivities of KCl solutions, with and without the addition of mannitol. The dielectrophoretic response of the capsids was recorded by measuring the frequency corresponding to zero dielectrophoretic force. The data were analysed using a multi-shelled model, and the permittivity and conductivity of the particles estimated. The capsid was modelled as a porous protein shell through which suspending medium passes, an inner chamber containing suspending medium in equilibrium with the outside, and a central core of protein (the scaffold). Capsids suspended in KCl without mannitol exhibited a different behaviour to those suspended in KCl with mannitol.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Rixon, Dr Frazer
Authors: Hughes, M.P., Morgan, H., and Rixon, F.J.
College/School:College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
Journal Name:European Biophysics Journal with Biophysics Letters
Publisher:Springer-Verlag
ISSN:0175-7571
ISSN (Online):1432-1017

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