Characterisation of a pucBA deletion mutant from Rhodopseudomonas palustris lacking all but the pucBAd genes

Southall, J. , Henry, S. L., Gardiner, A. T., Roszak, A. W., Mullen, W. , Carey, A.-M., Kelly, S. M. , de Percin Northumberland, C. O. and Cogdell, R. J. (2018) Characterisation of a pucBA deletion mutant from Rhodopseudomonas palustris lacking all but the pucBAd genes. Photosynthesis Research, 135(1-3), pp. 9-21. (doi: 10.1007/s11120-017-0386-7) (PMID:28567613)

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Abstract

Rhodopseudomonas palustris is a species of purple photosynthetic bacteria that has a multigene family of puc genes that encode the alpha and beta apoproteins, which form the LH2 complexes. A genetic dissection strategy has been adopted in order to try and understand which spectroscopic form of LH2 these different genes produce. This paper presents a characterisation of one of the deletion mutants generated in this program, the pucBAd only mutant. This mutant produces an unusual spectroscopic form of LH2 that only has a single large NIR absorption band at 800 nm. Spectroscopic and pigment analyses on this complex suggest that it has basically a similar overall structure as that of the wild-type HL LH2 complex. The mutant has the unique phenotype where the mutant LH2 complex is only produced when cells are grown at LL. At HL the mutant only produces the LH1-RC core complex.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Henry, Dr Sarah and Kelly, Dr Sharon and Roszak, Dr Aleksander and Mullen, Dr Bill and Cogdell, Professor Richard and Southall, Mrs June and Gardiner, Dr Alastair and carey, Dr Anne-Marie
Authors: Southall, J., Henry, S. L., Gardiner, A. T., Roszak, A. W., Mullen, W., Carey, A.-M., Kelly, S. M., de Percin Northumberland, C. O., and Cogdell, R. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Photosynthesis Research
Publisher:Springer
ISSN:0166-8595
ISSN (Online):1573-5079
Published Online:31 May 2017
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Photosynthesis Research 135(1-3): 9-21
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
502612Photosynthetic Antenna Research Center (PARC)Richard CogdellUS Department of Energy (USDOE)DE-SC0001035RI MOLECULAR CELL & SYSTEMS BIOLOGY