Comparative study of osteogenic activity of multilayers made of synthetic and biogenic polyelectrolytes

Guduru, D., Niepel, M. S., Gonzalez-Garcia, C., Salmeron-Sanchez, M. and Groth, T. (2017) Comparative study of osteogenic activity of multilayers made of synthetic and biogenic polyelectrolytes. Macromolecular Bioscience, 17(8), 1700078. (doi:10.1002/mabi.201700078) (PMID:28547877)

[img]
Preview
Text
141839.pdf - Accepted Version

1MB

Abstract

Polyelectrolyte multilayer (PEM) coatings on biomaterials are applied to tailor adhesion, growth, and function of cells on biomedical implants. Here, biogenic and synthetic polyelectrolytes (PEL) are used for layer-by-layer assembly to study the osteogenic activity of PEM with human osteosarcoma MG-63 cells in a comparative manner. Formation of PEM is achieved with biogenic PEL fibrinogen (FBG) and poly-l-lysine (PLL) as well as biotinylated chondroitin sulfate (BCS) and avidin (AVI), while poly(allylamine hydrochloride) (PAH) and polystyrene sulfonate (PSS) represent a fully synthetic PEM used as a reference system here. Surface plasmon resonance measurements show highest layer mass for FBG/PLL and similar for PSS/PAH and BCS/AVI systems, while water contact angle and zeta potential measurements indicate larger differences for PSS/PAH and FBG/PLL but not for BCS/AVI multilayers. All PEM systems support cell adhesion and growth and promote osteogenic differentiation as well. However, FBG/PLL layers are superior regarding MG-63 cell adhesion during short-term culture, while the BCS/AVI system increases alkaline phosphatase activity in long-term culture. Particularly, a multilayer system based on affinity interaction like BCS/AVI may be useful for controlled presentation of biotinylated growth factors to promote growth and differentiation of cells for biomedical applications.

Item Type:Articles
Keywords:Biotechnology, materials chemistry, bioengineering, polymers and plastics, biomaterials.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel and Gonzalez Garcia, Dr Cristina
Authors: Guduru, D., Niepel, M. S., Gonzalez-Garcia, C., Salmeron-Sanchez, M., and Groth, T.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Macromolecular Bioscience
Publisher:Wiley
ISSN:1616-5187
ISSN (Online):1616-5195
Published Online:26 May 2017
Copyright Holders:Copyright © 2017 Wiley-VCH Verlag GmbH & Co.
First Published:First published in Macromolecular Bioscience 17(8): 1700078
Publisher Policy:Reproduced in accordance with the publisher copyright policy

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
626901HEALINSYNERGY - Material-driven fibronectin fibrillogenesis to engineer synergistic growth factor microenvironmentsManuel Salmeron-SanchezEuropean Research Council (ERC)306990ENG - BIOMEDICAL ENGINEERING