Modelling foetal exposure to maternal smoking using hepatoblasts from pluripotent stem cells

Lucendo-Villarin, B., Filis, P., Swortwood, M. J., Huestis, M. A., Meseguer-Ripolles, J., Cameron, K., Iredale, J. P., O'Shaughnessy, P. , Fowler, P. A. and Hay, D. C. (2017) Modelling foetal exposure to maternal smoking using hepatoblasts from pluripotent stem cells. Archives of Toxicology, 91(11), pp. 3633-3643. (doi: 10.1007/s00204-017-1983-0) (PMID:28510779) (PMCID:PMC5696490)

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Abstract

The liver is a dynamic organ which is both multifunctional and highly regenerative. A major role of the liver is to process both endo and xenobiotics. Cigarettes are an example of a legal and widely used drug which can cause major health problems for adults and constitute a particular risk to the foetus, if the mother smokes during pregnancy. Cigarette smoke contains a complex mixture of thousands of different xenobiotics, including nicotine and polycyclic aromatic hydrocarbons. These affect foetal development in a sex-specific manner, inducing sex-dependant molecular responses in different organs. To date, the effect of maternal smoking on the foetal liver has been studied in vitro using cell lines, primary tissue and animal models. While these models have proven to be useful, poor cell phenotype, tissue scarcity, batch-to-batch variation and species differences have led to difficulties in data extrapolation toward human development. Therefore, in this study we have employed hepatoblasts, derived from pluripotent stem cells, to model the effects of xenobiotics from cigarette smoke on human hepatocyte development. Highly pure hepatocyte populations (>90%) were produced in vitro and exposed to factors present in cigarette smoke. Analysis of ATP levels revealed that, independent of the sex, the majority of smoking derivatives tested individually did not deplete ATP levels below 50%. However, following exposure to a cocktail of smoking derivatives, ATP production fell below 50% in a sex-dependent manner. This was paralleled by a loss metabolic activity and secretory ability in both female and male hepatocytes. Interestingly, cell depletion was less pronounced in female hepatocytes, whereas caspase activation was ~twofold greater, indicating sex differences in cell death upon exposure to the smoking derivatives tested.

Item Type:Articles
Additional Information:The project was funded by awards from the Medical Research Council MR/L010011/1, MR/K026666/1 and MR/L022974/1. <br>A correction to this article can be found at http://dx.doi.org/10.1007/s00204-017-2030-x.</br>
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:O'Shaughnessy, Professor Peter
Authors: Lucendo-Villarin, B., Filis, P., Swortwood, M. J., Huestis, M. A., Meseguer-Ripolles, J., Cameron, K., Iredale, J. P., O'Shaughnessy, P., Fowler, P. A., and Hay, D. C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Archives of Toxicology
Publisher:Springer
ISSN:0340-5761
ISSN (Online):1432-0738
Published Online:16 May 2017
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Archives of Toxicology 91(11): 3633-3643
Publisher Policy:Reproduced under a Creative Commons License
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