Kaye, K., Bryant, D. E., Marriott, K. E.R., Ohara, S., Fishwick, C. W.G. and Kee, T. P. (2016) Selective phosphonylation of 5′-adenosine monophosphate (5′-AMP) via pyrophosphite [PPi(III)]. Origins of Life and Evolution of Biospheres, 46(4), pp. 425-434. (doi: 10.1007/s11084-016-9497-y) (PMID:27220497) (PMCID:PMC5050244)
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Abstract
We describe here experiments which demonstrate the selective phospho-transfer from a plausibly prebiotic condensed phosphorus (P) salt, pyrophosphite [H2P2O52−; PPi(III)], to the phosphate group of 5′-adenosine mono phosphate (5′-AMP). We show further that this P-transfer process is accelerated both by divalent metal ions (M2+) and by organic co-factors such as acetate (AcO−). In this specific case of P-transfer from PPi(III) to 5′-AMP, we show a synergistic enhancement of transfer in the combined presence of M2+ & AcO−. Isotopic labelling studies demonstrate that hydrolysis of the phosphonylated 5′-AMP, [P(III)P(V)-5′-AMP], proceeds via nuceophilic attack of water at the Pi(III) terminus.
Item Type: | Articles |
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Additional Information: | The authors thank the EPSRC (grant EP/F042558/1), the Leverhulme Trust (grant F07112AA) and the UK Space Agency (Aurora Fellowship to TPK, 2009-12) for financial support of this work. This work was also supported by COST Action TD 1308 ORIGINS (life-origins.com). |
Keywords: | Astrobiology, phosphorus, prebiotic chemistry, origin of life, nucleotides. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Marriott, Miss Katie |
Authors: | Kaye, K., Bryant, D. E., Marriott, K. E.R., Ohara, S., Fishwick, C. W.G., and Kee, T. P. |
College/School: | College of Science and Engineering > School of Chemistry |
Journal Name: | Origins of Life and Evolution of Biospheres |
Publisher: | Springer |
ISSN: | 0169-6149 |
ISSN (Online): | 1573-0875 |
Published Online: | 24 May 2016 |
Copyright Holders: | Copyright © 2016 The Authors |
First Published: | First published in Origins of Life and Evolution of Biospheres 46(4): 425-434 |
Publisher Policy: | Reproduced under a Creative Commons License |
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